With the GO Enrichment feature in Partek Genomics Suite, you can take a list of significantly expressed genes/transcripts and find GO terms that are significantly enriched within the list. For a detailed introduction to GO Enrichment, refer to the GO Enrichment User Guide (Help > On-line Tutorials > User Guides).
Select the Diff_Exp_and_Alt_Splice spreadsheet from the spreadsheet tree
Select Gene Set Analysis in the Biological Interpretation section of the RNA-Seq workflow (Figure 1)
Figure 1. Selecting Gene Set Analysis
Select GO Enrichment in the Gene Set Analysis dialog (Figure 2)
Select Next >
Figure 2. Selecting the method of analysis
Select the spreadsheet 1/Diff_Exp_and_Alt_Splice (Diff Exp and Alt Splice.txt) from the drop-down menu (Figure 3)
Select Next >\
Figure 3. Selecting the spreadsheet that contains the genes you want to test
Select Use Fisher's Exact test
Select Invoke gene ontology browser on the result
Set Restrict analysis to functional groups with more than _ genes to 2 (Figure 4)
Select Next >
Figure 4. GO Enrichment options
Select Default mapping file (Figure 5)
Select Next >
Figure 5. Selecting the mapping file
A GO-Enrichment spreadsheet, as well as a browser (Figure 6), will be generated with the enrichment score shown for each GO term. Browse through the results to find a functional group of interest by examining the enrichment scores. The higher the enrichment score, the more over represented this functional group is in the input gene list. Alternatively, you may use the Interactive filter on the GO-Enrichment spreadsheet to identify functional groups that have low p-values and perhaps a higher percentage of genes in the group that are present.
Figure 6. Viewing the Gene Ontology Browser
If you need additional assistance, please visit our support page to submit a help ticket or find phone numbers for regional support.
