For the complete documentation index, see llms.txt. This page is also available as Markdown.
Get targeted mRNA
On a miRNA list, in order to perform biological interpretation, the targeted mRNA with gene symbol information is needed - gene symbol is used to map to the pathway database
On a data node contains miRNA list, e.g. differential analysis result data, Biological interpretation > Get targeted mRNA task will be enabled.
Database like TargetScan can be used to get targeted mRNAs for a specific mRNA. Specify the database in the dialog, and click Finish.
Double click on the Targeted mRNA node to open the report
Context++ score: estimating the strength of repression, more negative values suggest stronger predicted repression, this is the key metric for ranking predicted targets
Context++ score percentile: percentile ranking of the score compared to all predictions, higher percentile means stronger predicted effect
Weighted Context++ score: adjusted score considering multiple sites in the same transcript
Predicted relative KD: predicted relative dissociation constant is to measure the affinity between miRNA and targeted mRNA, lower KD means stronger binding affinity, higher KD means weaker binding.
