# Analysis and Data Management

In the **Analyses** tab, you'll see all the analyses in your study. Multiple analyses can be performed on each study, user can use sample group to create a subset of the data to perform analysis, or choose different analysis type: default or custom to analyze the data in a study.

### Card View vs List View

There are two types of display on *Studies* page and *Analyses* page. On the upper-right corner of the page, you can switch between the two view styles, card view vs list view.

<div align="left"><figure><img src="/files/8CuWrhLHxy2usyXOPQs4" alt="" width="563"><figcaption></figcaption></figure></div>

In **Card View**, each card will display the following information.

| Information   | Description                                                                                               |
| ------------- | --------------------------------------------------------------------------------------------------------- |
| Analysis Name | Name of analysis.                                                                                         |
| Status        | Status of analysis. See [Analysis Statuses](#analysis-statuses) for a table of statuses and descriptions. |
| Analysis Type | Type of omic data used in the default or custom analysis.                                                 |
| Modified By   | Last user who modified the analysis.                                                                      |
| Date Modified | Date and time when the last update was made.                                                              |

There are more controls in **List view**:

<div align="left"><figure><img src="/files/7zFiaSyvY2LK7aZRxSRm" alt="" width="563"><figcaption></figcaption></figure></div>

On column header, there is filter icon (![](/files/TseOqWNneIbzcwockGTL)), it allows to filter the rows in the table based on the selected value:

<div align="left"><figure><img src="/files/evljTGrZEokElxWWC3DC" alt="" width="449"><figcaption></figcaption></figure></div>

Click on the 3 dots next to the filter icon (![](/files/S9Vz5TeEJLKWBW3werlh)) to sort the table, choose columns to display

<div align="left"><figure><img src="/files/0Ynmlt7iDoh8yAAyVQCm" alt="" width="366"><figcaption></figcaption></figure></div>

### Create Analysis

To create a new analysis, click <img src="/files/Yos3m8v7S5TynedCftDa" alt="" data-size="line">.

Give your analysis a name and choose an analysis type from the dropdown menu.

### Analysis Options

| Analysis Option                                            | Description                                                                                                                                                                                                                                                                                                                                                                                                                                                 |
| ---------------------------------------------------------- | ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- |
| Default: Illumina Proteomics                               | General analysis pipeline for Illumina protein prep samples, with PCA and hierarchical clustering heatmap results. The maximum samples for analysis is 9,000.                                                                                                                                                                                                                                                                                               |
| Default: Illumina Single Cell Transcriptomics              | <p>General analysis pipeline for Illumina single cell prep samples, including the processing steps to output PCA and UMAP plots, plus graph-based clustering visualizations.</p><p>For each sample, all features are reported and if feature ID's are not unique, Mean is used for Deduplication; raw counts are used as the count value format and cells with a total read count at least 400 are reported. The maximum samples for analysis is 1,000.</p> |
| Default: Illumina Single Cell Transcriptomics -Perturb-seq | General analysis pipeline for Illumina single cell prep samples, including the processing steps to output PCA and UMAP plots, plus graph-based clustering visualizations. gRNAs are available but not further analyzed since user input is required for clustering and differential expression to obtain desired results. The maximum samples for analysis is 1,000.                                                                                        |
| Default: Illumina Spatial Transcriptomics                  | General analysis pipeline for Illumina spatial samples, including a Spatial map with transcripts overlayed on the tissue image where each point is a grid. Graph-based clusters are also plotted on a UMAP and pie chart. The maximum samples for analysis is 100.                                                                                                                                                                                          |
| Default: Illumina Bulk Transcriptomics                     | General analysis pipeline for Illumina mRNA and Total RNA prep samples including PCA and hierarchical clustering heatmap results. The maximum samples for analysis is 500.                                                                                                                                                                                                                                                                                  |
| Default: Illumina miRNA                                    | General analysis pipeline for Illumina miRNA prep samples including PCA and hierarchical clustering heatmap results. The maximum samples for analysis is 500.                                                                                                                                                                                                                                                                                               |
| Default: Illumina 5-base DNA Methylation                   | General analysis pipeline for 5-base DNA prep samples including regional methylation PCA plots, k-means clustering, and QC. The maximum samples for analysis is 200.                                                                                                                                                                                                                                                                                        |
| Custom: Multiomics                                         | Available inputs include Illumina proteomics, single cell, spatial, and bulk transcriptomics, miRNA, 5-base DNA and Infinium methylation as well as third-party data types including Seurat (RNA), Seurat (ATAC), Somalogic ADAT, Gene counts in sf Format, and VCF. Two or more data types can be analyzed together in any combination. The maximum samples for analysis is 200.                                                                           |
| Custom: Illumina Proteomics                                | Starts with the quantified samples which have undergone prior normalization and offers flexible analysis options. The maximum samples for analysis is 9,000.                                                                                                                                                                                                                                                                                                |
| Custom: Illumina Single Cell Transcriptomics               | Starts with single cell counts and offers flexibility with the analyses pipeline step. For each sample, all features are reported and if feature ID's are not unique, Mean is used for Deduplication; raw counts are used as the count value format and cells with a total read count at least 400 are reported. The maximum samples for analysis is 1,000.                                                                                                 |
| Custom: Illumina Single Cell Transcriptomics -Perturb-seq  | Starts with single cell counts and offers flexibility with the analyses pipeline step. For each sample, all features, both GEX and gRNAs, are reported and if feature ID's are not unique, Mean is used for Deduplication; raw counts are used as the count value format and cells with a total read count at least 400 are reported. The maximum samples for analysis is 1,000.                                                                            |
| Custom: Illumina Spatial Transcriptomics                   | Two starting nodes as cell-binned or grid-binned data, including the spatial image outputs, and offers flexibility with the analyses parameters. The maximum samples for analysis is 100.                                                                                                                                                                                                                                                                   |
| Custom: Illumina Bulk Transcriptomics                      | Starts with salmon format sample counts that have not been normalized and offers flexible analyses options. The assembly and annotation model used in secondary analysis is required to annotate the features. The maximum samples for analysis is 500.                                                                                                                                                                                                     |
| Custom: Illumina miRNA                                     | Starts with imported count matrix that have not been normalized and offers flexible analysis options. The maximum samples for analysis is 500.                                                                                                                                                                                                                                                                                                              |
| Custom: Illumina 5-base DNA Methylation                    | Starts with importing selected 5-base methylation data and offers flexible analysis options. The maximum samples for analysis is 200.                                                                                                                                                                                                                                                                                                                       |
| Custom: Illumina Infinium Methylation                      | Starts with importing selected idat files and offers flexible analysis options. No limit.                                                                                                                                                                                                                                                                                                                                                                   |
| Custom: Third-party analysis                               | Starts with data import. This option appears only when third-party assay data has been uploaded.                                                                                                                                                                                                                                                                                                                                                            |

Next, select the sample, sample group, or all samples for the analysis.

{% hint style="warning" %}
If all samples are chosen for the analysis, a sample group will be automatically added to the Sample Groups menu containing all of the samples in the analysis.
{% endhint %}

{% hint style="warning" %}
Current spatial multisample analysis does not yet support the import of a mix of samples that use pipeline-manifest.json and samples that do not. When running an analysis on a sample group that has both types of samples, only those samples with a pipeline-manifest.json file will import.
{% endhint %}

### Run Analysis

Click <img src="/files/PWSxNI8XFatqswIa1Def" alt="" data-size="line"> to run the analysis. You will receive a notification that the analysis was successfully created.

<figure><img src="/files/9HNszxBJUS9yQbjAqdxT" alt=""><figcaption></figcaption></figure>

Wait for the analysis status to change from "Pending" to "Complete".

<figure><img src="/files/vsFckjMj7T0cez8Nk4iQ" alt=""><figcaption></figcaption></figure>

### Analysis Statuses

The following table describes the different analysis statuses.

<table><thead><tr><th width="193">Status</th><th>Description</th></tr></thead><tbody><tr><td>Pending</td><td>The analysis has been initiated but has not yet started. It is waiting in the queue to be processed.</td></tr><tr><td>In Progress</td><td>The analysis is currently being executed. The system is processing the data and generating results.</td></tr><tr><td>Complete</td><td>The analysis has finished, and the results are ready to be viewed. Click into the analysis to view it.</td></tr><tr><td>Importing</td><td>The analysis is importing from a shared copy. The status will change to Complete upon successful import.</td></tr><tr><td>Offline</td><td>During import, the source server might be temporarily unavailable. Click the refresh button. Contact tech support if the issue persists.</td></tr><tr><td>Error</td><td>The analysis has ended in error. Using the Analysis tab list view, click the info icon <img src="/files/CTob3vWY9LiKlE6TeIEo" alt="" data-size="line">to get more information about the error type. The error can be further diagnosed using the <strong>Inspect analysis</strong> link.</td></tr></tbody></table>

Once the analysis is complete, you can click into it to view the results. Refer to the "[**Enter Analysis**](/icm/analyses/enter-analysis.md)" section for more information on how to view your analysis.

## Data

In the **Data** tab, you'll see a list of all the data and metadata files within your study from which your samples are derived.

{% hint style="warning" %}
Note that deleting data or metadata files will not remove any samples that were added to Connected Multiomics from those files. The samples will remain in Connected Multiomics even if the original files are deleted, ensuring that your sample data stays intact.
{% endhint %}

<div align="left"><figure><img src="/files/epe2IWV0efP0Aw1ntgIX" alt="" width="563"><figcaption></figcaption></figure></div>

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