On-Prem DRAGEN Server Analysis

This page describes details on running Illumina TruPath Genome analysis on prem via DRAGEN Server.

Running DRAGEN Germline in proximity mode for use with the Illumina TruPath Genome workflow via local on prem server will require a phase 4 server with upgraded compute support. For more information on v4 servers, please visit this link: DRAGEN secondary analysis on-premises server

A sample sheet is required for on-prem analysis with BCL Convert. A sample sheet is a comma-separated value (*.csv) file format used by Illumina instruments, platforms, and analysis pipelines to store settings and data for sequencing and analysis. DRAGEN Germline is compatible with the sample sheet v2. For general information on the sample sheet v2 format, refer to Illumina Connected Software - Sample Sheet.

The sample sheet includes a list of samples, along with additional information required to run DRAGEN Germline. When running analysis on a standalone DRAGEN server or on ICA, a valid sample sheet can be created by:

• BaseSpace Run Planner (preferred), see Sample Sheet Creation in BaseSpace Run Planner page for details, and refer to the BSSH Run Planning link to download a sample sheet for on-prem analysis.

• Downloading and modifying a sample sheet template following the requirements, see Sample Sheet Requirements page for details

The sample sheet for Planned runs can be downloaded by selecting the planned run and File -> Download -> SampleSheet.

For the most up to date information on the command lines required to run DRAGEN Germline for use with the Illumina TruPath Genome workflow, please navigate to the recipes section of the DRAGEN user guide here.

The sample sheet for Planned runs can be downloaded by selecting the planned run and File -> Download -> SampleSheet.