# Release notes

#### DRAGEN 16S Plus app version 1.0.0

Initial release.

**Component versions**

* Reference database: [Refseq-RDP-v1](https://zenodo.org/records/2541239) (full-length NCBI RefSeq 16S rRNA database supplemented by RDP)
* Analysis Pipeline version: 0.7.0
* DRAGEN version: 4.4.4-19

**Known Issues**

* Analysis fails when launched with a single-read Fastq file
* When Read QC is disabled, the "Classification Statistics" table values and "Top Results by Taxonomic Level” barplots are missing from sample HTML report files
* When reading Biosamples from a Project, Fastq files for Biosamples sharing the same sample name prefix before the first underscore are merged. For example, Fastq files for Biosamples PREFIX\_001 and PREFIX\_002 will be merged and reported as a single PREFIX sample. To avoid this error, ensure that sample names are unique before the first underscore, replace underscores with a hyphen, or provide Biosample input from a list

**Known Limitations**

* 100 basepairs minimum read length
* 500 million basepairs maximum for custom database specification
* Species level classification from 16S amplicon data is generally less reliable than genus level classification
* Summary dendrogram chart may not be rendered
* Summary species rarefaction curve chart may not be rendered
* Sample-level species rarefaction curve chart may not be rendered