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DRAGEN Protein Quantification
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DRAGEN Protein Quantification
  • Get Started
    • Introduction
    • Prerequisites
      • Illumina Protein Prep Automation System Output Files
  • Run Setup
    • Run Planning with the BSSH Run Planner Tool
    • Sample Sheet Fields
    • Local Sample Sheet Generation Tool
    • Lane Splitting and Multi-Analysis by Project
  • Counting and Normalization
    • Cloud Autolaunch Secondary Analysis
      • Accessing Cloud Results
    • Local Secondary Analysis
    • Normalization Summary
    • QC Summary
    • Interpretation of Results
    • Metrics Appendix
  • Output Files
    • Output Structure
    • DRAGEN Report
    • ADAT Content
  • After Counting and Normalization
    • Using the ADAT
    • Illumina Connected Multiomics Walkthrough
  • References
    • FAQs
    • Documentation Revision History
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  1. Get Started

Introduction

NextPrerequisites

Last updated 1 month ago

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End to End (E2E) (IPP) workflow combines Illumina chemistry, SOMAmer technology, and DRAGEN data analysis for a comprehensive, automated NGS-based proteomics solution. This E2E solution provides the following:

  • NGS readout of more than 9K unique protein targets for a single plasma or serum sample.

  • From sample to processed results in under 2.5 days with just 4 hours of hands-on time.

  • Integrated analysis via (BSSH), (ICA), and Illumina Connected Multiomics (powered by ).

    • Includes both local and cloud solutions for planning a run and for processing data with DRAGEN Protein Quantification.

E2E Overview

The Illumina E2E Protein Prep solution integrates automation steps to perform sample preparation, protein capture, sequencing, and bioinformatics analysis. Once sequencing is finished, the DRAGEN Protein Quantification application automatically initiates in BSSH or ICA. The diagram below illustrates the E2E workflow.

For documentation on the assay and automation components of Illumina Protein Prep, please refer to the Illumina Protein Prep Product Documentation (document # 200045446).

Illumina Protein Prep
BaseSpace Sequence Hub
Illumina Connected Analytics
Partek
E2E Illumina Protein Prep (IPP) workflow.