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DRAGEN Protein Quantification
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DRAGEN Protein Quantification
  • Get Started
    • Introduction
    • Prerequisites
      • Illumina Protein Prep Automation System Output Files
  • Run Setup
    • Run Planning with the BSSH Run Planner Tool
    • Sample Sheet Fields
    • Local Sample Sheet Generation Tool
    • Lane Splitting and Multi-Analysis by Project
  • Counting and Normalization
    • Cloud Autolaunch Secondary Analysis
      • Accessing Cloud Results
    • Local Secondary Analysis
    • Normalization Summary
    • QC Summary
    • Interpretation of Results
    • Metrics Appendix
  • Output Files
    • Output Structure
    • DRAGEN Report
    • ADAT Content
  • After Counting and Normalization
    • Using the ADAT
    • Illumina Connected Multiomics Walkthrough
  • References
    • FAQs
    • Documentation Revision History
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  • Plate QC
  • Sample QC
  • Specification

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  1. Output Files

DRAGEN Report

DRAGEN Reports is an HTML report that provides a quick overview of the quality of an E2E analysis. The report consists of three sections, which are displayed as tabs in the report. The following sections describe the tabs.

Plate QC

This section is subdivided into the following subsections:

  • Reagent Lot Summary: This table describes the reagents used per plate in the analysis.

  • Plate QC Summary: This table provides the plate level metrics, including Calibration % in Tails, QC % in Tails, Reference Correlation, and Blank Background metrics.

  • Calibration Scale Factors: This histogram illustrates the distribution of calibration scale factors for a given plate.

  • QC Scale Factors: This histogram illustrates the distribution of QC scale factors for a given plate.

Sample QC

This section of the report contains information on which samples passed or flagged specifications as well as SOMAmer count yield per sample. It is subdivided into the following subsections:

  • Sample QC Summary: The table describes the percentage of samples (organized by sample type) that passed Quality Control.

  • QC Summary: The heatmap shows the QC status of samples based on their position in the plate wells.

  • Flagged Samples: The table identifies samples that failed one or more sequencing or normalization specifications.

  • SOMAmer Read Counts: This graph represents the SOMAmer count for each sample included in the analysis.

Specification

This report section details the QC metrics and normalization steps.

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