Prerequisites

• NovaSeq 6000/6000Dx, NextSeq 2000, or NovaSeq X Series

• Illumina Single Cell 3' RNA Prep Kit

  • T2: 1 library per sample, 1 FASTQ pair

  • T10: 1 library per sample, 1 FASTQ pair

  • T20: 1 library per sample, 1 FASTQ pair

  • T100: 4 libraries per sample, 1 FASTQ pair

  • T1000: 8 libraries per sample, 8 FASTQ pairs

  • For more information about the library preparation kit, refer to the Illumina Single Cell Prep Support Site.

• A cloud account with a valid subscription. For information on registering your BaseSpace Sequence Hub or Illumina Connected Analytics account, refer to Software Registration page.