Bulk RNA-Seq Analysis Training

Agenda with start times:

• Transfer file to the server (00:02:28)

• Import fastq files (00:03:55)

• Add sample attributes (00:06:00)

• Pre-alignment QA/QC (00:10:45)

• Alignment QC (00:15:30)

• Quantification (00:21:45)

• Filter and normalization (00:32:13)

• Differential analysis and visualization (00:38:50)

• Filter genes to generate a heatmap (00:53:50)

• Biological interpretation (01:08:00)