Illumina® DRAGEN™ ORA Decompression Software Family

Warning This user guide is intended for users who are working with FASTQ.ORA files AND who are not using the DRAGEN secondary analysis software.

DRAGEN v3.8 has introduced ORA Compression, a fully lossless compression algorithm to compress FASTQ.GZ files at a ratio up to x5 vs FASTQ.GZ. FASTQ.ORA files can be used as-is within the DRAGEN ecosystem. For users who are not using the DRAGEN secondary analysis software, FASTQ.ORA files can either be:

• used as-is, in a seamless manner, with most common downstream software, using DRAGEN ORA Helper Suite standalone software; or

• decompressed, using DRAGEN ORA Decompression standalone software.

These two standalone software can be downloaded for free, from the ORA Support Site

Please refer to the relevant user guide for using them.