# Single Cell Toolkit System Requirements * [Up to 100,000 cells per analysis](#up-to-100000-cells-per-analysis) * [More than 100,000 cells per analysis](#more-than-100000-cells-per-analysis) Because of the large size of single cell RNA-Seq data sets and the computationally-intensive tools used in single cell analysis, we have amended our system requirements and recommendations for installations of Partek Flow with the Single Cell toolkit. ## Up to 100,000 cells per analysis #### Required * Linux: Ubuntu® 18.04, Redhat® 8, CentOS® 8, or newer * CPU: 64-bit 2 GHz quad-core processor * Memory: 64 GB of RAM * Local scratch space\*: 1 TB with cached or native speeds of 2GB/s or higher * Storage: > 2 TB available for data and > 100 GB on the root partition #### Recommended * Linux: Ubuntu® 18.04, Redhat® 8, CentOS® 8, or newer * CPU: 64-bit 2 GHz quad-core processor * Memory: 128 GB of RAM * Local scratch space1: 2 TB with cached or native speeds of 2GB/s or higher * Storage: > 2 TB available for data and > 100 GB on the root partition ## More than 100,000 cells per analysis #### Required * Linux: Ubuntu® 18.04, Redhat® 8, CentOS® 8, or newer * CPU: 64-bit 2 GHz quad-core processor * Memory: 256 GB of RAM * Local scratch space1: 2 TB with cached or native speeds of 2GB/s or higher * Storage: > 4 TB available for data #### Recommended * Linux: Ubuntu® 18.04, Redhat® 8, CentOS® 8, or newer * CPU: 64-bit 2 GHz quad-core processor * Memory: 512 GB of RAM * Local scratch space1: 10 TB with cached or native speeds of 2GB/s or higher * Storage: 10 TB available for data For fastest performance: * Newer generation CPU cores with avx2 or avx-512 are recommended. * Performance scales proportionality to the number of CPU cores available. * Hyper thread cores (threads) scales performance for most operations other than principal component analysis. *\*Contact Partek support for recommended setup of local scratch storage* ## Additional Assistance If you need additional assistance, please visit [our support page](http://www.partek.com/support) to submit a help ticket or find phone numbers for regional support. --- # Agent Instructions: Querying This Documentation If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question. Perform an HTTP GET request on the current page URL with the `ask` query parameter: ``` GET https://help.connected.illumina.com/partek/partek-flow/installation-guide/single-cell-toolkit-system-requirements.md?ask= ``` The question should be specific, self-contained, and written in natural language. The response will contain a direct answer to the question and relevant excerpts and sources from the documentation. Use this mechanism when the answer is not explicitly present in the current page, you need clarification or additional context, or you want to retrieve related documentation sections.