# Running post-alignment QA/QC

After alignment has completed, we can view the quality of alignment by performing post-alignment QA/QC.

* Click the **Aligned reads** data node
* Click **QA/QC** inthe task menu
* Click **Post-alignment QA/QC** from the QA/QC section of the task menu (Figure 1)

<figure><img src="https://1384254481-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FJVEESmJAPppJ3ijFq5aR%2Fuploads%2Fgit-blob-149ac6da15cd2809f5ade2653e963b50d02f374d%2Fselect%20post-alignment%20QAQC.png?alt=media" alt=""><figcaption><p><em>Figure 1. Invoking Post-alignment QA/QC</em></p></figcaption></figure>

A Post-alignment QA/QC task node will be generated (Figure 2).

<figure><img src="https://1384254481-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FJVEESmJAPppJ3ijFq5aR%2Fuploads%2Fgit-blob-eb14defcad77fe05416b2c4d8d0112bbe1e2da45%2Fpost-alignment%20QAQC%20task%20node.png?alt=media" alt=""><figcaption><p><em>Figure 2. Post-alignment QA/QC task node</em></p></figcaption></figure>

* Double-click the **Post-alignment QA/QC** task node to view the task report

Similar to the Pre-alignment QA/QC task report, general quality information about the whole data set is displayed and sample-level reports can be opened by clicking a sample name in the table.

The first two graphs in the report (Figure 3) show the alignment breakdown and total reads in each sample.

<figure><img src="https://1384254481-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FJVEESmJAPppJ3ijFq5aR%2Fuploads%2Fgit-blob-a7349c37ca9201ab5705c45dc98e8e038f7bba1c%2Falignment-statistics-for-the-data-set.png?alt=media" alt=""><figcaption><p><em>Figure 3. Alignment statistics for the data set</em></p></figcaption></figure>

From these graphs, we can see that more than 95% of reads were aligned, but the total number of reads for each sample varies. Normalizing for the variability in total read counts will be addressed in a later section of the tutorial.

For more information about the graphs and information presented in the Post-alignment QA/QC task report, see the [Post-alignment QA/QC](https://help.connected.illumina.com/partek/partek-flow/user-manual/task-menu/qa-qc/post-alignment-qa-qc) user guide.

## Additional Assistance

If you need additional assistance, please visit [our support page](http://www.partek.com/support) to submit a help ticket or find phone numbers for regional support.
