Visualizing gene expression in Chromosome view

From the DESeq2 task report, we can browse to any gene in the Chromosome view.

• Click in the KLHDC7B row to open Chromosome view (Figure 1)

Figure 1. Browsing to a location in Chromosome View

A new tab will open showing KLHDC7B in the Chromosome view (Figure 2).

Figure 2. Viewing in Chromosome view

Chromosome View shows reference genome, annotation, and data set information together aligned at genomic coordinates.

Each track has Configure track and Move track buttons that can be used to modify each track.

The top track shows average number of total count normalized reads for each of the three treatment groups in a stacked histogram. The second track shows the Ensembl annotation.

We can add tracks from any data node using Select Tracks.

• Click Select tracks

A pop-up dialog showing the pipeline allows us to choose which data to display as tracks in Chromosome view (Figure 3).

Figure 3. Choosing tracks to display in Chromosome view

• Click Reads pileup under Aligned reads on the left-hand side of the dialog

• Click Display selection to make the change

The reads pileup track is now included (Figure 4).

Figure 4. The reads pileup track shows every read in its aligned position on the reference genome

To learn more about Chromosome view, please consult the Chromosome View user guide.

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