# Kruskal-Wallis * [Running the task](#running-the-task) * [Report](#report) The Kruskal-Wallis and Dunn's tests (Non-parametric ANOVA) task is used to identify deferentially expressed genes among two or more groups. Note that such rank-based tests are generally advised for use with larger sample sizes. ## Running the task To invoke the Kruskal-Wallis test, select any count-based data nodes, these include: * Gene counts * Transcript counts * Normalized counts Select *Statistics > Differential analysis* in the context-sensitive menu, then select *Kruskal-Wallis* (Figure 1).

Figure 1. Select any count node to invoke the Non-parametric ANOVA task

Select a specific factor for analysis and click the **Next** button (Figure 2). Note that this task can only take into account one factor at a time.

Figure 2. Select one factor for analysis

For more complicated experimental designs, go back to the original count data that will be used as input and perform **Rank normalization** at the *Features* level (Figure 3). The resulting *Normalized counts* data node can then be analyzed using the **Detect differential expression (ANOVA)** task, which can take into account multiple factors as well as interactions.

Figure 3. Normalize your count data by rank to do non-parametric testing on more complicated experimental designs

Define the desired comparisons between groups and click the **Finish** button (Figure 4). Note that comparisons can only be added between single group (i.e. one group per box).

Figure 4. Set-up desired comparisons

## Report The results of the analysis will appear similar to other differential expression analysis results. However, the column to indicate mean expression levels for each group will display the median instead (Figure 5).

Figure 5. The task's ANOVA report will display the median instead of the LSmean

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