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Illumina GenomeStudio Plugin

The GenomeStudio plug-in lets you export data into a project that can be opened in Partek Genome Suite open directly. It is the fastest and most consistent way to get fully annotated Illumina data into Partek Genomics Suite.

  • Import gene expression data

  • Import Genotype Data

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Additional Assistance

If you need additional assistance, please visit to submit a help ticket or find phone numbers for regional support.

Import Genotype Data

This user guide describes how to export copy number and genotype data using Partek's Report Plug-in for Illumina GenomeStudio Genotype Module for use in Partek Genome Suite. The GenomeStudio plug-in lets you export data into a project that can be opened in Partek Genome Suite open directly. It is the fastest and most consistent way to get fully annotated Illumina gene expression data into Partek.

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Partek Genotype plug-in installation

Download the plug-in zip file

unzip the file, there is a folder called PartekReport which contains two .dll files --Partek.Common.dll

Export CNV data to Illumina GenomeStudio using Partek report plug-in
Import data from Illumina GenomeStudio using Partek plug-in
Export methylation data to Illumina GenomeStudio using Partek report plug-in
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and
Partek.GeneExpression.GenomeStudio.dll
, move the
PartekReport
folder to

C:\Program Files \Illumina\GenomeStudio 2.0\Modules\BSGT\ReportPlugins, if there is no ReportPlugins folder in BSGT folder, create one, the path and folder names have to be exactly match one described above (Figure 1).

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Export report from GenomeStudio

In GenomeStudio genotype project:

  • Choose Analysis > Reports>Report Wizard from the main menu

  • Select Custom Report and choose Partek Report Plug-in from the drop-down list

  • Specify AnnotationName, do NOT include <> in the name, you can the same name as the .bgx file you imported the ddata with, or a unique name to your dataset

Figure 1. Configuring the GenomeStudio copy number report dialog

  • Leave all the others as default value (Figure 2) click Next

  • Specify the report file name, we recoommend to put the exported files in their own folder, which allows you to move thefolder instead of all the files individually.

  • Click Finish (Figure 2)

Figure 2. Specify output folder and file name

The output generate 9 files in the folder including a project file (.ppj), annotation file, summary file and 3 sets of Partek spreadshet file-- each spreadsheet consists of 2 files.

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Open project in Partek Genomics Suite

To open the report, launch Partek Genomics Suite, choose File > Open Project, browse to the .ppj file to open. There will be three spreadsheets opened (Figure 3)

Figure 3. Open project in Partek Genomics Suite

Spreadsheet 1 contains genotype calls, spreadsheet 2 contains log R ratio which is copy number in log scale, spreadsheet 3 contains B allele frequency.

To do copy number analysis, select spreadsheet 2 log R ratio, choose Copy number workflow, start from QA/QC section. Genotype spreadsheet will be used for Association and LOH workflow.

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Additional Assistance

If you need additional assistance, please visit to submit a help ticket or find phone numbers for regional support.

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PartekReportGX.zip
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Export methylation data to Illumina GenomeStudio using Partek report plug-in

This document was developed for Partek Genomics Suite version 6.6 software. Documentation for Partek Genomics Suite version 7.0 software is in development and will replace this document.

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GenomeStudioMethylationPlugin.pdf
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Additional Assistance

If you need additional assistance, please visit our support pagearrow-up-right to submit a help ticket or find phone numbers for regional support.

Import gene expression data

This user guide describes how to export gene expression data using Partek's Report Plug-in for Illumina GenomeStudio Gene Expression Module for use in Partek Genome Suite. The GenomeStudio plug-in lets you export data into a project that can be directly opened in Partek Genomics Suite. It is the fastest and most consistent way to get fully annotated Illumina gene expression data into Partek Genomics Suite.

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Partek Gene Expression plug-in installation

Download the plug-in zip file

file-archive
55KB
PartekReportGX.zip
archive
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unzip the file, there is a folder called PartekReport which contains two .dll files --Partek.Common.dll and Partek.GeneExpression.GenomeStudio.dll, move the PartekReport folder to

C:\Program Files (x86)\Illumina\GenomeStudio\Modules\BSGX\ReportPlugins, if there is no ReportPlugins folder in BSGX folder, create one, the path and folder names have to be exactly match one described above (Figure 1).

Figure 1. Place PartekReport folder in the appropriate direcotry

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Export report from GenomeStudio

In GenomeStudio gene expression project:

  • Choose Analysis > Reports... from the main menu

  • Select Custom Report and choose Partek Report Plug-in from the drop-down list

  • Specify AnnotationName, do NOT include <> in the name, you can the same name as the .bgx file you imported the data with, or a unique name to your dataset

Figure 2. Configuring the GenomeStudio gene expression report dialog

There are five files exported, including a project file (.ppj), which can be opened directory in Partek Genomic Suite. The project file opens the signal intensities data in a spreadsheet and associates the annotation information to the intensity spreadsheet. All intensities are log2 transformed. If there are negative values in the AVG_Signal, the data will be shifted to the lowest value one and then log2 transformed.

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Open project in Partek Genomics Suite

To open the report, launch Partek Genomics Suite, choose File > Open Project, browse to the .ppj file to open. In the Gene Expression workflow, you can proceed add sample attribute step.

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Additional Assistance

If you need additional assistance, please visit to submit a help ticket or find phone numbers for regional support.

Import data from Illumina GenomeStudio using Partek plug-in

This document was developed for Partek Genomics Suite version 6.6 software. Documentation for Partek Genomics Suite version 7.0 software is in development and will replace this document.

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Additional Assistance

If you need additional assistance, please visit to submit a help ticket or find phone numbers for regional support.

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GenomeStudioGenotypePlugin.pdf
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Choose Type by clicking on the cell, default is gene level

  • Leave all the others as default value (Figure 2)

  • Specify the report file name, we recommend to put the exported files in their own folder, which allows you to move the folder instead of all the files individually.

  • Click OK

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    Export CNV data to Illumina GenomeStudio using Partek report plug-in

    This document was developed for Partek Genomics Suite version 6.6 software. Documentation for Partek Genomics Suite version 7.0 software is in development and will replace this document.

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    Additional Assistance

    If you need additional assistance, please visit to submit a help ticket or find phone numbers for regional support.

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    GenomeStudioGeneExpressionPlugin.pdf
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