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  1. Run Set Up
  2. Sample Sheet
  3. Index Orientation Guide

i5 Index Orientation Table

This table describes how to enter the i5 sequence, Forward (F) or Reverse Complement (RC), depending on the instrument and software used.

  • LRM: Local Run Manager

  • IEM: Illumina Experiment Manager

  • BSSH: BaseSpace Sequence Hub

  • IRM: Illumina Run Manager

  • Manual Edit: When manually preparing the Sample Sheet in the text editor

Instrument

i5 Index Orientation Used Based on Run Setup Tool

Standalone bcl2fastq or BSSH FASTQ Generation

Standalone BCL Convert

MiSeq

F (LRM, IEM)

F

F

F (LRM, Preptab, IEM)

RC

RC

iSeq 100

F (LRM, IEM)

RC

RC

NextSeq 500/550

F (LRM, Preptab, IEM)

RC

RC

NextSeq 1000/2000

F (Manual Edit, BSSH Run Planning)

RC

F

NovaSeq 6000 (v1.5 kits)

RC (Manual Edit), F (BSSH Run Planning)

RC

RC

NovaSeq X/X Plus

F (IRM, BSSH Run Planning)

RC

F

MiSeq i100 Series

F (IRM, BSSH Run Planning)

F (index-first) RC (read-first)

F

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Last updated 4 months ago

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