i5 Index Orientation Table
This table describes how to enter the i5 sequence, Forward (F) or Reverse Complement (RC), depending on the instrument and software used.
Instrument
i5 Index Orientation Used Based on Run Setup Tool
Standalone bcl2fastq or BSSH FASTQ Generation
Standalone BCL Convert
MiSeq
F (LRM, IEM)
F
F
F (LRM, Preptab, IEM)
RC
RC
iSeq 100
F (LRM, IEM)
RC
RC
NextSeq 500/550
F (LRM, Preptab, IEM)
RC
RC
NextSeq 1000/2000
F (Manual Edit, BSSH Run Planning)
RC
F
NovaSeq 6000 (v1.5 kits)
RC (Manual Edit), F (BSSH Run Planning)
RC
RC
NovaSeq X/X Plus
F (IRM, BSSH Run Planning)
RC
F
MiSeq i100 Series
F (IRM, BSSH Run Planning)
F (index-first) RC (read-first)
F
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