# i5 Index Orientation Table

This table describes how to enter the i5 sequence, Forward (F) or Reverse Complement (RC), depending on the instrument and software used.

{% hint style="info" %}

* LRM: Local Run Manager
* IEM: Illumina Experiment Manager
* BSSH: BaseSpace Sequence Hub
* IRM: Illumina Run Manager
* Manual Edit: When manually preparing the Sample Sheet in the text editor
  {% endhint %}

<table data-header-hidden data-full-width="true"><thead><tr><th></th><th width="329"></th><th></th><th></th></tr></thead><tbody><tr><td><strong>Instrument</strong></td><td><strong>i5 Index Orientation Used Based on Run Setup Tool</strong></td><td><strong>Standalone bcl2fastq or BSSH FASTQ Generation</strong></td><td><strong>Standalone BCL Convert</strong></td></tr><tr><td>MiSeq</td><td>F (LRM, IEM)</td><td>F</td><td>F</td></tr><tr><td><a data-footnote-ref href="#user-content-fn-1">MiniSeq</a></td><td>F (LRM, Preptab, IEM)</td><td>RC</td><td>RC</td></tr><tr><td>iSeq 100</td><td>F (LRM, IEM)</td><td>RC</td><td>RC</td></tr><tr><td>NextSeq 500/550</td><td>F (LRM, Preptab, IEM)</td><td>RC</td><td>RC</td></tr><tr><td>NextSeq 1000/2000</td><td>F (Manual Edit, BSSH Run Planning)</td><td>RC</td><td>F</td></tr><tr><td>NovaSeq 6000 (v1.5 kits)</td><td>RC (Manual Edit), F (BSSH Run Planning)</td><td>RC</td><td>RC</td></tr><tr><td>NovaSeq X/X Plus</td><td>F (IRM, BSSH Run Planning)</td><td>RC</td><td>F</td></tr><tr><td>MiSeq i100 Series</td><td>F (IRM, BSSH Run Planning)</td><td>F (index-first)<br>RC (read-first)</td><td>F</td></tr></tbody></table>

[^1]: MiniSeq Rapid Kits: Always forward orientation


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