# NextSeq 1000/2000 Sample Sheet Version History * :white\_check\_mark: = Supported * :yellow\_square: = Supported. Change in definition from prior release. See Release Notes and/or Sample Sheet Definition for each version for details. * Blank = Not supported

Pipeline	Section	Type	Key Word	3.5.8	3.7.4	3.8.4	3.9.3	3.10.10	3.10.12	4.2.7
DRAGEN BCL Convert	[BCLConvert_Settings]	Required	SoftwareVersion	✅	✅	✅	✅	✅	✅	✅
DRAGEN BCL Convert	[BCLConvert_Settings]	Optional	OverrideCycles	✅	✅	✅	✅	✅	✅	✅
DRAGEN BCL Convert	[BCLConvert_Settings]	Optional	AdapterRead1	✅	✅	✅	✅	✅	✅	✅
DRAGEN BCL Convert	[BCLConvert_Settings]	Optional	AdapterRead2	✅	✅	✅	✅	✅	✅	✅
DRAGEN BCL Convert	[BCLConvert_Settings]	Optional	FastqCompressionFormat		✅	✅	✅	✅	✅	✅
DRAGEN BCL Convert	[BCLConvert_Settings]	Optional	NoLaneSplitting							✅
DRAGEN BCL Convert	[BCLConvert_Data]	Required	SampleID	✅	✅	✅	✅	✅	✅	✅
DRAGEN BCL Convert	[BCLConvert_Data]	Optional	Index	✅	✅	✅	✅	✅	✅	✅
DRAGEN BCL Convert	[BCLConvert_Data]	Optional	Index2	✅	✅	✅	✅	✅	✅	✅
DRAGEN Germline	[DragenGermline_Settings]	Required	SoftwareVersion	✅	✅	✅	✅		✅	✅
DRAGEN Germline	[DragenGermline_Settings]	Required	ReferenceGenomeDir	✅	✅	✅	✅		✅	✅
DRAGEN Germline	[DragenGermline_Settings]	Optional	MapAlignOutFormat	✅	✅	✅	✅		✅	✅
DRAGEN Germline	[DragenGermline_Settings]	Optional	KeepFastq		✅	✅	✅		✅	✅
DRAGEN Germline	[DragenGermline_Data]	Required	SampleID	✅	✅	✅	✅		✅	✅
DRAGEN Enrichment	[DragenEnrichment_Settings]	Required	SoftwareVersion	✅	✅	✅	✅		✅	✅
DRAGEN Enrichment	[DragenEnrichment_Settings]	Required	ReferenceGenomeDir	✅	✅	✅	✅		✅	✅
DRAGEN Enrichment	[DragenEnrichment_Settings]	Optional	MapAlignOutFormat	✅	✅	✅	✅		✅	✅
DRAGEN Enrichment	[DragenEnrichment_Settings]	Required	BedFile	✅	✅	✅	✅		✅	✅
DRAGEN Enrichment	[DragenEnrichment_Settings]	Required	GermlineOrSomatic		✅	✅	✅		✅	✅
DRAGEN Enrichment	[DragenEnrichment_Settings]	Optional	KeepFastq		✅	✅	✅		✅	✅
DRAGEN Enrichment	[DragenEnrichment_Settings]	Optional	AuxNoiseBaselineFile			✅	✅		✅	✅
DRAGEN Enrichment	[DragenEnrichment_Settings]	Optional	AuxCnvPanelOfNormalsFile						✅	✅
DRAGEN Enrichment	[DragenEnrichment_Data]	Required	SampleID	✅	✅	✅	✅		✅	✅
DRAGEN RNA	[DragenRna_Settings]	Required	SoftwareVersion	✅	✅	✅	✅		✅	✅
DRAGEN RNA	DragenRna_Settings]	Required	ReferenceGenomeDir	✅	✅	✅	✅		✅	✅
DRAGEN RNA	DragenRna_Settings]	Optional	RnaGeneAnnotationFile	✅	✅	✅	✅		✅	✅
DRAGEN RNA	DragenRna_Settings]	Optional	MapAlignOutFormat	✅	✅	✅	✅		✅	✅
DRAGEN RNA	DragenRna_Settings]	Optional	KeepFastq		✅	✅	✅		✅	✅
DRAGEN RNA	DragenRna_Settings]	Optional	DifferentialExpressionEnable			✅	✅		✅	✅
DRAGEN RNA	[DragenRna_Data]	Required	SampleID	✅	✅	✅	✅		✅	✅
DRAGEN RNA	[DragenRna_Data]	Optional	Comparison1			✅	✅		✅	✅
DRAGEN RNA	[DragenRna_Data]	Optional	Comparison2			✅	✅		✅	✅
DRAGEN RNA	[DragenRna_Data]	Optional	Comparison3			✅	✅		✅	✅
DRAGEN RNA	[DragenRna_Data]	Optional	Comparison4			✅	✅		✅	✅
DRAGEN RNA	[DragenRna_Data]	Optional	Comparison5			✅	✅		✅	✅
DRAGEN Single Cell	[DragenSingleCellRna_Settings]	Required	SoftwareVersion		✅	✅	✅		✅	✅
DRAGEN Single Cell	[DragenSingleCellRna_Settings]	Required	ReferenceGenomeDir		✅	✅	✅		✅	✅
DRAGEN Single Cell	[DragenSingleCellRna_Settings]	Optional	MapAlignOutFormat		✅	✅	✅		✅	✅
DRAGEN Single Cell	[DragenSingleCellRna_Settings]	Optional	RnaGeneAnnotationFile		✅	✅	✅		✅	✅
DRAGEN Single Cell	[DragenSingleCellRna_Settings]	Optional	KeepFastq		✅	✅	✅		✅	✅
DRAGEN Single Cell	[DragenSingleCellRna_Settings]	Required	BarcodePosition		✅	✅	✅		✅	✅
DRAGEN Single Cell	[DragenSingleCellRna_Settings]	Required	UmiPosition		✅	✅	✅		✅	✅
DRAGEN Single Cell	[DragenSingleCellRna_Settings]	Required	BarcodeRead		✅	✅	✅		✅	✅
DRAGEN Single Cell	[DragenSingleCellRna_Settings]	Required	BarcodeSequenceWhitelist		✅
DRAGEN Single Cell	[DragenSingleCellRna_Settings]	Required	BarcodeSequenceList			✅	✅		✅	✅
DRAGEN Single Cell	[DragenSingleCellRna_Settings]	Required	RnaLibraryType		✅	✅	✅		✅	✅
DRAGEN Single Cell	[DragenSingleCellRna_Data]	Required	SampleID		✅	✅	✅		✅	✅
DRAGEN Amplicon	[DragenAmplicon_Settings]	Required	SoftwareVersion			✅	✅		✅	✅
DRAGEN Amplicon	[DragenAmplicon_Settings]	Required	ReferenceGenomeDir			✅	✅		✅	✅
DRAGEN Amplicon	[DragenAmplicon_Settings]	Required	DnaBedFile			✅	✅		✅	✅
DRAGEN Amplicon	[DragenAmplicon_Settings]	Optional	MapAlignOutFormat			✅	✅		✅	✅
DRAGEN Amplicon	[DragenAmplicon_Settings]	Optional	KeepFastq			✅	✅		✅	✅
DRAGEN Amplicon	[DragenAmplicon_Settings]	Required	DnaGermlineOrSomatic			✅	✅		✅	✅
DRAGEN Amplicon	[DragenAmplicon_Data]	Required	DnaOrRna			✅	✅		✅	✅
DRAGEN Amplicon	[DragenAmplicon_Data]	Required	SampleID			✅	✅		✅	✅
Nanostring	[DragenGeoMxNGS_Settings]	Required	SoftwareVersion				✅			✅
Nanostring	[DragenGeoMxNGS_Settings]	Optional	KeepFastq				✅			✅
Nanostring	[DragenGeoMxNGS_Settings]	Required	GeoMxConfigFile				✅			✅
Nanostring	[DragenGeoMxNGS_Settings]	Required	BarcodeSequenceList				✅			✅
Nanostring	[DragenGeoMxNGS_Data]	Required	SampleID				✅			✅