Auto-Launch of DRAGEN TSO 500 ctDNA Analysis on ICA

Auto-launch Prerequisites and Workflow

*The BaseSpace Sequence Hub setting for run monitoring and storage must be selected on the instrument to use DRAGEN TSO 500 ctDNA analysis auto-launch. For information on preparing your instrument for DRAGEN TSO 500 ctDNA Auto-launch, refer to the documentation for your instrument.

1. Use BaseSpace Sequence Hub Run Planning tool or the sample sheet templates provided on the support page to create and export a sample sheet.

   1. If BaseSpace Run Planning tool is not available in your region, use the sample sheet template.

2. Import the sample sheet to the instrument and start the sequencing run. Refer to ICA Auto-launch Sample Sheet Requirements for sample sheet guidance.

   1. Data is uploaded to BaseSpace Sequence Hub and then pushed to ICA. You can monitor the run in BaseSpace Sequence Hub.

   2. Analysis auto launches in ICA when sequencing and the upload completes. You can monitor the status of the analysis in BaseSpace Sequence Hub or ICA

   3. If necessary, you can requeue the analysis via BaseSpace Sequence Hub.

3. View the analysis output results in either BaseSpace Sequence Hub or ICA.

To avoid invalid sample sheet configurations, Illumina recommends using BaseSpace Run Planning tool to generate sample sheets. Using an invalid sample sheet can result in failed runs and analyses.

BaseSpace Sequence Hub Requirements for ICA Auto-Launch

BaseSpace Run Planning tool is a multi-step workflow that generates a manual launch or auto-launch capable sample sheet for export and requires the following additional settings:

• Access to BaseSpace Sequence Hub.

• ICA Run Storage is enabled under BaseSpace Sequence Hub settings.

Refer to the BaseSpace Sequence Hub support site page for information on setting up a BaseSpace Sequence Hub project.

Requeue Analysis

You can requeue analysis of a run via the run's Summary page in BaseSpace Sequence Hub.

Refer to the BaseSpace Sequence Hub support site page for more information on requeuing an analysis.

Minimum Storage Requirements on ICA

Sequencing System	Minimum Disk Space (Gb)
NovaSeq 6000/6000Dx (RUO) SP Flow Cell	500
NovaSeq 6000/6000Dx (RUO) S1 Flow Cell	1100
NovaSeq 6000/6000Dx (RUO) S2 Flow Cell	2500
NovaSeq 6000/6000Dx (RUO) S4 Flow Cell	4300
NovaSeq X 1.5B	2000
NovaSeq X 10B	4300
NovaSeq X 25B	6000
NextSeq 2000 P4 Flow Cell	500

Refer to the Software Registration page for information on how to manage accounts and subscriptions.

Guided Examples

Please review these guided examples of using DRAGEN TSO 500 Analysis Software with auto-launch on ICA:

• NovaSeq 6000Dx: TSO 500 Auto-launch Analysis in Cloud