Analysis Launch on ICA

Methods for Launching Analysis

Illumina Connected Analytics (ICA) supports the following methods for launching DRAGEN TruSight Oncology 500 Analysis Software.

• Auto-launch—Stream run data directly from the instrument to ICA via a specially configured sample sheet and automatically begin DRAGEN TSO 500 analysis.

• Manual launch—Initiate DRAGEN TSO 500 analysis on ICA using the run files and sample sheet files in the project.

For more information about using ICA or BaseSpace Sequence Hub, refer to the following support pages on the Illumina support site.

• Illumina Connected Analytics support site page

• BaseSpace Sequence Hub support site page

Resources used by ICA Analyses

EC2 Instance Support

As of DRAGEN TSO500 v2.6.2, ICA pipelines are able to run on F2 instance type nodes (in addition to F1, when available).

For additional details on performance and availability of supported instance types, please see: F2 Support on ICA

BCL Convert in parallel by lane

As of DRAGEN TSO500 v2.6.2, BCL Convert in ICA pipeline is parallelized by lane. A node is created to convert BCL to fastq from a particular lane. After demultiplex is complete for all lanes, the fastq files are then aggregated per sample. On average, the turn-around time for BCL Convert per lane is ~35mins.