Sample Sheet Preparation for 5B Flow Cell

Prepare a sample sheet for a NovaSeq X 5B flow cell.

Data generated by 5B flow cell (NovaSeq X) is only compatible with DRAGEN TSO 500 analysis software v2.6.2

NovaSeq X 5B flow cells use a two-step analysis workflow:

1. Generate FASTQ files with BCL Convert v4.4.10.
2. Run variant calling with DRAGEN TSO 500 v2.6.2 from FASTQ files.

To create a sample sheet to enable 5B data analysis, follow the steps below.

1

Create and export the sample sheet

1. Follow Sample Sheet Creation in BaseSpace Run Planning tool.
2. Set Starts from Fastq to True.
3. Export the generated sample sheet.

2

Update the BCL Convert pipeline URN

1. Open the exported file in a text editor.
2. Use Find and Replace to replace this value:

urn:ilmn:ica:pipeline:a0778b16-f318-40df-ae04-95998e3a7564#BCL_Convert_v3_10_9_for_TSO500

with this value:

urn:ilmn:ica:pipeline:4bdae602-abe4-4230-b0c6-f886af563b26#BclConvert_v4_4_10

1. Save the modified sample sheet.

3

Use the updated file

Upload the updated sample sheet to the pre-planned sequencing run on NovaSeq X.