# Sample Sheet Templates

DRAGEN TSO 500 analysis software is compatible with several instruments and assay workflows (standard, XP), each of which have implications for the sample sheet.

Sample sheet templates contain all required fields, including index sequences in the proper orientation for all indexes from a given library prep kit. The templates are provided as a starting point for creating a sample sheet manually when launching analysis on a standalone DRAGEN server or on ICA using manual launch.

{% hint style="info" %}
For interactive run planning or to create a sample sheet for ICA Autolaunch, use [BaseSpace Run Planner](https://basespace.illumina.com/) to create valid sample sheets for either local or cloud analysis. To set up a run in BaseSpace run planner, refer to [Sample Sheet Creation in BaseSpace Run Planner](https://help.connected.illumina.com/tso500/dragen-tso-500-guides/dragen-tso-500-v2.6/run-planning/sample-sheet-creation-in-basespace-run-planning-tool).
{% endhint %}

Users can visit the [Sample Sheet guidelines](https://help.connected.illumina.com/tso500/dragen-tso-500-guides/dragen-tso-500-v2.6/run-planning/sample-sheet-requirements) section to learn additional details on required fields and values as they fill-in their sample information. Use the lookup table below to select and download the sample sheet template that matches your instrument, assay, and workflow configuration:

<table data-full-width="true"><thead><tr><th width="185.15234375">Assay</th><th width="282.234375">Instrument</th><th width="128.4140625">Assay Workflow</th><th data-type="files">Files</th></tr></thead><tbody><tr><td>TSO500</td><td>NextSeq 550</td><td>Standard</td><td><a href="https://3845108255-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F7XRWgkRPkhoHXVslBqXD%2Fuploads%2Fgit-blob-b5c503f650cdb1b2f27ef4c66545e07bc8b91165%2FSample_Sheet_Template_TSO%20500_i5Rev_Std_Local.csv?alt=media">Sample_Sheet_Template_TSO 500_i5Rev_Std_Local.csv</a></td></tr><tr><td>TSO500 + HRD</td><td>NextSeq 550</td><td>Standard</td><td><a href="https://3845108255-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F7XRWgkRPkhoHXVslBqXD%2Fuploads%2Fgit-blob-eb8ff066dafc8119e8dea89c6b315aa0b2315b12%2FSample_Sheet_Template_TSO%20500%20HRD_i5Rev_Std_Local.csv?alt=media">Sample_Sheet_Template_TSO 500 HRD_i5Rev_Std_Local.csv</a></td></tr><tr><td>TSO500 + HRD</td><td>NovaSeq 6000</td><td>Standard</td><td><a href="https://3845108255-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F7XRWgkRPkhoHXVslBqXD%2Fuploads%2Fgit-blob-eb8ff066dafc8119e8dea89c6b315aa0b2315b12%2FSample_Sheet_Template_TSO%20500%20HRD_i5Rev_Std_Local.csv?alt=media">Sample_Sheet_Template_TSO 500 HRD_i5Rev_Std_Local.csv</a></td></tr><tr><td>TSO500 + HRD</td><td>NovaSeq 6000Dx (in RUO mode)</td><td>Standard</td><td><a href="https://3845108255-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F7XRWgkRPkhoHXVslBqXD%2Fuploads%2Fgit-blob-57b8dfd7e6c6deb863698a138c6d1d2b8e4025c2%2FSample_Sheet_Template_TSO%20500%20HRD_i5Fwd_Std_Local.csv?alt=media">Sample_Sheet_Template_TSO 500 HRD_i5Fwd_Std_Local.csv</a></td></tr><tr><td>TSO500 HT</td><td>NextSeq 1000 / 2000</td><td>Standard</td><td><a href="https://3845108255-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F7XRWgkRPkhoHXVslBqXD%2Fuploads%2Fgit-blob-b87f0cccadf5ea0742f67bea6d699f54932a61f5%2FSample_Sheet_Template_TSO%20500%20HT_i5Fwd_Std_Local.csv?alt=media">Sample_Sheet_Template_TSO 500 HT_i5Fwd_Std_Local.csv</a></td></tr><tr><td>TSO500 HT</td><td>NovaSeq 6000</td><td>Standard</td><td><a href="https://3845108255-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F7XRWgkRPkhoHXVslBqXD%2Fuploads%2Fgit-blob-34cb2c13c2deea39cd387a14adf4f589f5f807a4%2FSample_Sheet_Template_TSO%20500%20HT_i5Rev_Std_Local.csv?alt=media">Sample_Sheet_Template_TSO 500 HT_i5Rev_Std_Local.csv</a></td></tr><tr><td>TSO500 HT</td><td>NovaSeq 6000</td><td>XP*</td><td><a href="https://3845108255-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F7XRWgkRPkhoHXVslBqXD%2Fuploads%2Fgit-blob-ca03d77ac38158861c43676ee7630d45b25ea50f%2FSample_Sheet_Template_TSO%20500%20HT_i5Rev_XP_Local.csv?alt=media">Sample_Sheet_Template_TSO 500 HT_i5Rev_XP_Local.csv</a></td></tr><tr><td>TSO500 HT</td><td>NovaSeq 6000Dx (in RUO mode)</td><td>Standard</td><td><a href="https://3845108255-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F7XRWgkRPkhoHXVslBqXD%2Fuploads%2Fgit-blob-b87f0cccadf5ea0742f67bea6d699f54932a61f5%2FSample_Sheet_Template_TSO%20500%20HT_i5Fwd_Std_Local.csv?alt=media">Sample_Sheet_Template_TSO 500 HT_i5Fwd_Std_Local.csv</a></td></tr><tr><td>TSO500 HT</td><td>NovaSeq 6000Dx (in RUO mode)</td><td>XP*</td><td><a href="https://3845108255-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F7XRWgkRPkhoHXVslBqXD%2Fuploads%2Fgit-blob-9649dc055217c02b518e9f7cc2ca086b10fcf686%2FSample_Sheet_Template_TSO%20500%20HT_i5Fwd_XP_Local.csv?alt=media">Sample_Sheet_Template_TSO 500 HT_i5Fwd_XP_Local.csv</a></td></tr><tr><td>TSO500 HT</td><td>NovaSeq X</td><td>Standard</td><td><a href="https://3845108255-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F7XRWgkRPkhoHXVslBqXD%2Fuploads%2Fgit-blob-b87f0cccadf5ea0742f67bea6d699f54932a61f5%2FSample_Sheet_Template_TSO%20500%20HT_i5Fwd_Std_Local.csv?alt=media">Sample_Sheet_Template_TSO 500 HT_i5Fwd_Std_Local.csv</a></td></tr><tr><td>TSO500 HT</td><td>NovaSeq X</td><td>XP*</td><td><a href="https://3845108255-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F7XRWgkRPkhoHXVslBqXD%2Fuploads%2Fgit-blob-9649dc055217c02b518e9f7cc2ca086b10fcf686%2FSample_Sheet_Template_TSO%20500%20HT_i5Fwd_XP_Local.csv?alt=media">Sample_Sheet_Template_TSO 500 HT_i5Fwd_XP_Local.csv</a></td></tr><tr><td>TSO500 v2</td><td>NextSeq 550/DX (in RUO Mode)</td><td>Standard</td><td><a href="https://3845108255-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F7XRWgkRPkhoHXVslBqXD%2Fuploads%2Fgit-blob-7a0704cef6b06a534827cb56091356ca8a181afa%2FSample_Sheet_Template_TSO_500_HRD_v2_i5Rev_Std_Autolaunch.csv?alt=media">Sample_Sheet_Template_TSO_500_HRD_v2_i5Rev_Std_Autolaunch.csv</a></td></tr><tr><td>TSO500 v2</td><td>NextSeq 1000 / 2000</td><td>Standard</td><td><a href="https://3845108255-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F7XRWgkRPkhoHXVslBqXD%2Fuploads%2Fgit-blob-64c33225198ca5623ae36943f76b4e53f4677aef%2FSample_Sheet_Template_TSO_500_HRD_v2_i5Fwd_Std_Autolaunch.csv?alt=media">Sample_Sheet_Template_TSO_500_HRD_v2_i5Fwd_Std_Autolaunch.csv</a></td></tr><tr><td>TSO500 v2</td><td>NovaSeq 6000</td><td>Standard</td><td><a href="https://3845108255-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F7XRWgkRPkhoHXVslBqXD%2Fuploads%2Fgit-blob-7a0704cef6b06a534827cb56091356ca8a181afa%2FSample_Sheet_Template_TSO_500_HRD_v2_i5Rev_Std_Autolaunch.csv?alt=media">Sample_Sheet_Template_TSO_500_HRD_v2_i5Rev_Std_Autolaunch.csv</a></td></tr><tr><td>TSO500 v2</td><td>NovaSeq 6000</td><td>XP*</td><td><a href="https://3845108255-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F7XRWgkRPkhoHXVslBqXD%2Fuploads%2Fgit-blob-82973ef177ed091a985eeacf0792d9d5c28f53c8%2FSample_Sheet_Template_TSO_500_HRD_v2_i5Rev_XP_Autolaunch.csv?alt=media">Sample_Sheet_Template_TSO_500_HRD_v2_i5Rev_XP_Autolaunch.csv</a></td></tr><tr><td>TSO500 v2</td><td>NovaSeq 6000Dx (in RUO mode)</td><td>Standard</td><td><a href="https://3845108255-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F7XRWgkRPkhoHXVslBqXD%2Fuploads%2Fgit-blob-64c33225198ca5623ae36943f76b4e53f4677aef%2FSample_Sheet_Template_TSO_500_HRD_v2_i5Fwd_Std_Autolaunch.csv?alt=media">Sample_Sheet_Template_TSO_500_HRD_v2_i5Fwd_Std_Autolaunch.csv</a></td></tr><tr><td>TSO500 v2</td><td>NovaSeq 6000Dx (in RUO mode)</td><td>XP*</td><td><a href="https://3845108255-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F7XRWgkRPkhoHXVslBqXD%2Fuploads%2Fgit-blob-f45efdd028115e0262102174ace2734845be0d37%2FSample_Sheet_Template_TSO_500_HRD_v2_i5Fwd_XP_Autolaunch.csv?alt=media">Sample_Sheet_Template_TSO_500_HRD_v2_i5Fwd_XP_Autolaunch.csv</a></td></tr><tr><td>TSO500 v2</td><td>NovaSeq X</td><td>Standard</td><td><a href="https://3845108255-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F7XRWgkRPkhoHXVslBqXD%2Fuploads%2Fgit-blob-64c33225198ca5623ae36943f76b4e53f4677aef%2FSample_Sheet_Template_TSO_500_HRD_v2_i5Fwd_Std_Autolaunch.csv?alt=media">Sample_Sheet_Template_TSO_500_HRD_v2_i5Fwd_Std_Autolaunch.csv</a></td></tr><tr><td>TSO500 v2</td><td>NovaSeq X</td><td>XP*</td><td><a href="https://3845108255-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F7XRWgkRPkhoHXVslBqXD%2Fuploads%2Fgit-blob-f45efdd028115e0262102174ace2734845be0d37%2FSample_Sheet_Template_TSO_500_HRD_v2_i5Fwd_XP_Autolaunch.csv?alt=media">Sample_Sheet_Template_TSO_500_HRD_v2_i5Fwd_XP_Autolaunch.csv</a></td></tr></tbody></table>