The following information provides the requirements and steps to install the DRAGEN ORA Helper Suite Software.
The following table lists the minimum requirements for the DRAGEN ORA Helper Suite Software.
Info The oraFuse software uses a FUSE file system. Because the FUSE file system requires root privileges, the oraFuse software requires root privileges during installation.\
Download the installer from the , and follow the installation steps below to install the DRAGEN ORA Helper Suite Software. Installation Steps:
1. Open a terminal window.
2. If the installation is for an online system, then move the installer to the directory where the software should be installed. If the installation is for an offline system, then the installer does not need to be moved.
3. Navigate to the installer location.
4. Run the following command:
sh <file name>.sh
5. To install oraFuse, install the dependencies when prompted. The following dependencies will automatically be installed on an online system:
Info If the installation is for an offline system, then make sure the dependencies are installed on the offline system.\
The installer creates a folder named oraHelperSuite. If the installation is for an offline system, then move the oraHelperSuite folder to the offline system. The oraHelperSuite folder contains the following:
orad executable
oraFuse executable
ora-ldpreload.so library
oraHelper wrapper executable
For decompression of FATSQ.ORA derived from specific species/models, the specific reference needs to be downloaded from the . The whole reference database can also be downloaded.
Info For default human reference, there is no need to add this extra step. The default human reference is already included in the DRAGEN ORA Decompression Software.\
1. Move the downloaded archive to the oraHelperSuite directory
mv yourdownloadpath/<genus_specificname>.tar.gz yourpath/oraHelperSuite
2. Navigate to the oraHelperSuite directory
cd yourpath/oraHelperSuite
3. Extract the archive file using the following command:
When the extraction of the specific reference is completed the oraHelperSuite folder on a linux environment should be structured as follows with example on gallus_gallus reference:
oraHelperSuite |__orad |__orafuse |__ora-ldpreload.so |__oraHelper |__refbin |__Gallus_gallus |__refbin |__readme_gallus_gallus
1. Move the downloaded archive to the oraHelperSuite directory
mv yourdownloadpath/oradata.tar.gz yourpath/oraHelperSuite
2. Navigate to the the oraHelperSuite directory
cd yourpath/oraHelperSuite
3. Extract the archive file using the following command:
When the extraction of the full database is completed the the oraHelperSuite folder on a linux environment should be structured as follows:
oraHelperSuite |__orad |__orafuse |__ora-ldpreload.so |__oraHelper |__refbin |__oradata |__Homo_sapiens |__refbin |__readme_homo_sapiens |__Homo_sapiens_bisulfite |__refbin |__readme_homo_sapiens_bisulfite |__Genus_specificname |__refbin |__readme_Genus_specificname
the human reference genome used to decompress: refbin. The pre-installed refbin is the default human reference. If the FASTQ.ORA file has been compressed with a different reference ensure the correct reference is available locally or download it from the ORA Support Site.
System memory
8 GB RAM
Free disk space
From 2 GB to 15 GB depending on how many different specific references are needed for the decompression. Specific references are available for downloads in the ORA Support Site. For human data, 2 GB are required. The default human reference is already pre-installed within the Software and does not need to be downloaded.
Compatible Linux distributions
CentOS 7 or later
Ubuntu 14 or later
Oracle 8 or later
Internet connection
An internet connection is required to install the DRAGEN ORA Helper Suite. Once installed, the internet connection is not required unless the DRAGEN ORA Helper Suite is used in a remote environment.
CentOS
fuse
fuse-libs
libcurl
openssl
Ubuntu
fuse
openssl
libcurl3-gnutls
Oracle
fuse
fuse-libs
fuse-devel
libcurl
tar -xzvf <genus_specificname>.tar.gz (Linux)
tar -xzvf <genus_specificname>.tar.gz (Mac)tar -xzvf oradata.tar.gz (Linux)
tar -xzvf oradata.tar.gz (Mac)