RNA-Seq alignment conversion to Correlation Engine Biosets

There are two options to transfer data to Correlation Engine from the outputs of the BaseSpace RNA-Seq alignment app. One, you can download the entire analysis in the project page.

1. Navigate to the FILES view in your analysis

2. Under the counts folder, select the *.genes file to download

1. Edit the file by adding the headers 'Gene name" and Test Expression to the first and second column respectively. Filter the data file to remove any rows with zero Test expression counts.

Gene name	Test expression
FAM41C	16
LOC100130417	43
SAMD11	228
NOC2L	590
KLHL17	33

Note:

1. When using gene symbol identifiers, check at the verification step during upload to make sure the correct species was assigned to your upload

2. Using unique refseq or ensembl identifiers will ensure the application recognizes the correct species

3. The import process will consider any rows above the column names as free-text space for any information the user wants to include about the data and will be viewable in the application