Adding sample attributes

Attributes describe samples. Examples of sample attributes include treatment group, age, sex, and time point. Attributes can be added individually in the Metadata tab or in bulk using a text file. In this tutorial, we will add one attribute, 5-AZA Dose, manually.

• Click the Metadata tab

• Click Manage under Sample attributes (Figure 1)

Figure 1. Adding sample attributes manually

• Click Add new attribute (Figure 2)

Figure 2. Adding a new attribute

• To configure a new attribute, at Name, type in 5-AZA Dose as the name of the attribute

• Click Add to add 5-AZA Dose as a categorical, project-specific attribute (Figure 3)

Figure 3. Configuring a new attribute

• Name the first New category 0uM

• Click the green plus icon to add category (Figure 4)

• Repeat for two additional categories, 5uM and 10uM (Figure 5)

Figure 4. Creating attribute category

Figure 5. All category attributes for 5-AZA Dose

• Click Back to metadata tab

The data table now includes an Attribute column for 5-AZA Dose (Figure 6). Next, we need to assign samples attribute categories for 5-AZA Dose.

Figure 6. Data table updated with column for 5-AZA Dose

• Select Assign values

The option to edit the 5-AZA Dose field for each sample will appear as a drop-down menu (Figure 7).

Figure 7. Dropdown menu to select treatment for each sample

• Select the 5-AZA Dose text box for a sample to bring up a drop-down menu with the 5-AZA Dose attribute categories (0uM, 5uM, 10uM)

• Use the drop-down menus to add a treatment group for each sample

The first three samples (SRR592573-5) should be 0uM, the next three samples (SRR592576-8) should be 5uM, and the final three samples (SRR592579-81) should be 10uM (Figure 8).

• Click Apply changes

Figure 8. Add 5-AZA dose to each sample as shown

The data table will now show each a 5-AZA Dose attribute for each sample.

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