Check Losslessness

DRAGEN ORA Compression is a lossless compression.

If you wish to verify that no data was lost during the compression of the fastq.ora file, compare the MD5 checksum of the decompressed fastq.ora file and the MD5 checksum of the decompressed fastq.gz file.

1. Compute the md5 checksum of the uncompressed FASTQ.ORA content as follows

md5sum <(orad myfile.fastq.ora --raw -c )

2. Compute the md5 checksum of the uncompressed FASTQ.GZ content as follows

md5sum <(gzip -d -c myfile.fastq.gz)