# Key Output Files and Metrics

Navigate to the completed analysis run to review the results.

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Results files per sample can be found within the analysis.

### Key Output Files

To learn more about key 5-Base specific output files, reference the DRAGEN documentation: <https://help.dragen.illumina.com/product-guide/dragen-v4.4/dragen-methylation-pipeline/dragen-5base-pipeline#methylation-calling-outputs>

To learn more about non-5-Base specific output files, reference the DRAGEN DNA Pipeline documentation: <https://help.dragen.illumina.com/product-guide/dragen-v4.4/dragen-dna-pipeline>

### Where to Start when Reviewing QC

A positive (pUC19) and negative (lambda) control genome are spiked-in to each sample during library prep. Neither control is fully methylated/unmethylated, but the pUC19 control should have over 90% methylation, and the lambda control should have under 1.5% methylation. To check the methylation for these controls, review the DRAGEN Report and navigate to the Methylation tab, and then the Context QC table. The "% of CpG" column reflects the % methylation of these controls.

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### CX Report

The \*CX\_report file will be generated for all analyses. It will have the following format:

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* Each line = 1 cytosine in reference genome (with non-zero coverage)​
* \# reads can be dedup’d or not, depending on analysis settings (we use dedupping)​
* mC=methylated, C=unmethylated support​
* Overall methylation level = mC\_reads / (mC\_reads + C\_reads)​
* Overall coverage (STRANDED) = mC\_reads + C\_reads
