# DRAGEN 16S Plus App Documentation

## ![](https://1177916873-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F4OEB7VheHjRG5hBZXuPi%2Fuploads%2Fgit-blob-67a17dac15ca25cc43da5018c0226d371a6e5cd0%2F16s_plus_app_logo.png?alt=media) Overview

### Summary: <a href="#summary" id="summary"></a>

The DRAGEN 16S Plus application is a rapid, kmer-based informatics solution designed for microbial classification and community profiling from mixed flora and metagenomic sample types. The app delivers easy-to-use, powerful secondary analysis of Illumina 16S sequencing data, with workflows for read QC (optional), taxonomic classification, result filtering (optional), and reporting. It also supports custom database analysis.

### Input files: <a href="#input-files" id="input-files"></a>

* FASTQ files
* [Custom database FASTA file](https://help.connected.illumina.com/infectious-disease/dragen-16s-plus/dragen-16s-plus/how-to-set-up-and-run-an-analysis/custom-database-fasta-file-format) (if applicable)

### Demo Data: <a href="#demo-data" id="demo-data"></a>

The [DRAGEN 16S Plus Demo Project](https://ilmn-sso.basespace.illumina.com/datacentral) includes 21 samples prepared using the [Illumina 16S metagenomic sequencing library preparation protocol](https://support.illumina.com/downloads/16s_metagenomic_sequencing_library_preparation.html). An example custom reference sequence FASTA file is also included.

### Analysis Pipeline: <a href="#analysis-pipeline" id="analysis-pipeline"></a>

1. **Read QC** (optional)
2. **Taxonomic classification**
3. **Result filters** (optional read count threshold)
4. **Reporting**

### Output files: <a href="#output-files" id="output-files"></a>

* Analysis-level outputs: **XLSX**, **CSV**, **HTML**, **ZIP**
* Sample-level outputs: **JSON**, **CSV**, **HTML**, **TXT.GZ**

### Important Notes: <a href="#important-notes" id="important-notes"></a>

DRAGEN 16S Plus is a secondary analysis tool for research use only. Further interpretation, statistical analysis, and downstream analysis of results may be necessary.

For questions on this application please contact Illumina Technical Support at <techsupport@illumina.com>.

#### For Research Use Only. Not for use in diagnostic procedures.