# Plan a NovaSeq X Series Run

Use the following instructions to plan a run for the NovaSeq X series systems in BaseSpace Sequence Hub.

1. Select the **Runs** tab, and then select the **New Run** drop-down.
2. Select **Run Planning**.
3. In the Run Name field, enter a unique name of your preference to identify the current run. The run name can contain a maximum of 255 alphanumeric characters, spaces, dashes, and underscores.
4. **\[Optional]** Enter a description for the run. The run description can contain a maximum of 255 characters.
5. Select your sequencing system as the instrument platform.
6. Select one of the following analysis locations.
   * **BaseSpace** — Analyze sequencing data in the cloud.
   * **DRAGEN Server** - Analyze sequencing data on a standalone DRAGEN server. When this option is selected, the planned run can only be exported to a sample sheet v2 file.
   * **Local** — Analyze sequencing data on-instrument. When this option is selected, the planned run can only be exported to a sample sheet v2 file.
7. Enter the number of cycles performed in each read: If using multiple analysis configurations, use the longest read length required by the configuration. When setting up a configuration, override automatically trims the length based on the recommended lengths for the selected library prep kit.
   * **Read 1** — Enter the number of cycles for Read 1.
   * **Index 1** — Enter the number of cycles for the Index 1 (i7) primer. For a PhiX-only run, enter 0 in both index fields.
   * **Index 2** — Enter the number of cycles for the Index 2 (i5) primer.
   * **Read 2** — Enter the number of cycles for Read 2.
8. **\[Optional]** Enter the ID for your library tube. The library tube ID is located on the label of your library tube strip.
9. Select **Next**.

> Please take note of the following when setting up a configuration.
>
> * Instrument Platform and Analysis location in Run Settings page are not editable once a Configuration is created.
> * Application version cannot be changed once a Configuration is saved. You need to delete the configuration and create a new one instead.

10. Select your analysis application.
11. **\[Optional]** Enter a description for the configuration.
12. Select a library prep kit or add a new custom library prep kit as follows.
    * Select **Add Custom Library Prep Kit** under the Library Prep Kit dropdown.
    * Enter the name, read types, default read cycles, and compatible index adapter kits for your custom library prep kit.
    * Select **Create New Kit**.
13. Select an index adapter kit or add a new a custom index kits as follows. If you are using more than one library, the libraries must have the same index read lengths.
    * Select **Add Custom Index Adapter Kit** under the Index Adapter Kit dropdown.
    * Select a template type and enter the kit name, adapter sequences, index strategies, and index sequences. Make sure the second index (i5) adapter sequences are in forward orientation.
    * Select **Create New Kit**.
14. If applicable to your application, select a reference genome.
15. Select **Next** to configure secondary analysis settings.