TruSeq DNA Exome v2.0
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The TruSeq DNA Exome v2.0 includes the following functionality:
Preconfigured TruSeq DNA Exome v2.0 protocol that supports the preparation of 96 indexed, paired-end libraries, followed by enrichment using reagents provided in an Illumina® TruSeq® Exome Kit.
Automated calculation of sample and buffer volumes.
Automated calculation or display of reagents at every step in the protocol.
Automatic step transition when required.
Automatic placement of samples when necessary.
Automated assignment of QC Pass/Fail, based on user-selected threshold values.
A routing script that allows sequencing of libraries to any Illumina sequencing instrument.
Protocol Type = Library Prep
Next Steps Configuration
Master Step Name = Normalize DNA (TruSeq DNA Exome v2.0.10)
Step Type = Standard
Derived Sample Generation = Fixed, 1
Naming Convention = {InputItemName}
Reagent Kits
TruSeq Exome Kit
Supplier = Illumina
Catalog Number = 24 - 20020614; 96 - 20020615
Website = www.illumina.com
The version of Normalize DNA master step name may be different depending on the version of IPP installed.
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Sample Table (Column Headers)
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Project
Project Name
Built-in
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Placement Pattern = Column
Destination Containers
96 well plate
Step Data (Master Step Fields)
Field Name
Field Type
Options
Additional Options and Dropdown Items
DNA Amount (ng)
Numeric
Read Only
Default = 100
EDTA (uL)
Numeric
Read Only
Default = 10
Notes
Multiline Text
Read Only
Default = DNA Amout (ng) and Total Volume (uL) are both per sample.
RSB (mL)
Numeric
Read Only
Default = 5
Total Volume (uL)
Numeric
Read Only
Default = 50
Decimal Places Displayed = 0
Step File Placeholders
Log - Automatically attached
Sample Table
Sample Display Default = Expand
Well Sort Order = Row
Table Columns - Global Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Concentration
Numeric
Required Field
Decimal Places Displayed = 2
Derived Sample
Conc. Units
Text
Required Field
Derived Sample
Sample Name
Built-in
Derived Sample
Sample Volume (uL)
Numeric
Decimal Places Displayed = 2
Derived Sample
Shearing Buffer Mix (uL)
Numeric
Decimal Places Displayed = 0
Project
Project Name
Built-in
Master Step Name = Fragment DNA (TruSeq Exome v1.0)
Step Type = Standard
Derived Sample Generation = Fixed, 1
Naming Convention = {SubmittedSampleName}
Reagent Kits
TruSeq Exome Kit
Supplier = Illumina
Catalog Number = 24 - 20020614; 96 - 20020615
Website = www.illumina.com
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Sample Table (Column Headers)
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Project
Project Name
Built-in
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Placement Pattern = Column
Destination Containers
Tube
96 well plate
Step Data (Master Step Fields)
Field Name
Field Type
Options
Additional Options and Dropdown Items
Comment
Multiline Text
Covaris Cycles/Burst
Numeric
Default = 200
Decimal Places Displayed = 0
Covaris Duration (seconds)
Numeric Dropdown
Custom Entries
Presets
375
280
360
420
Decimal Places Displayed = 0
Covaris Duty Factor (%)
Numeric Dropdown
Custom Entries
Presets
20
10
30
Decimal Places Displayed = 0
Covaris Intensifier
Text Dropdown
Custom Entries
Presets
Yes
No
Covaris Intensity
Numeric
Decimal Places Displayed = 0
Covaris Peak Power (W)
Numeric Dropdown
Custom Entries
Presets
50
175
450
Decimal Places Displayed = 0
Covaris Temperature (C)
Numeric Dropdown
Custom Entries
Presets
20
7
Decimal Places Displayed = 0
Covaris Water Level
Numeric Dropdown
Custom Entries
Presets
12
6
Decimal Places Displayed = 0
Step File Placeholders
Log File - Automatically attached
Sample Table
Sample Display Default = Expand
Well Sort Order = Row
Table Columns - Global Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Project
Project Name
Built-in
Master Step Name = TruSeq DNA Exome v2.0.10
Step Type = Standard
Derived Sample Generation = Fixed, 1
Naming Convention = {InputItemName}
Reagent Kits
TruSeq Exome Kit
Supplier = Illumina
Catalog Number = 24 - 20020614; 96 - 20020615
Website = www.illumina.com
The version of TruSeq DNA Exome master step name may be different depending on the version of IPP installed.
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Sample Table (Column Headers)
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Project
Project Name
Built-in
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Placement Pattern = Column
Destination Containers
Tube
96 well plate
Step Data (Master Step Fields)
Field Name
Field Type
Options
Additional Options and Dropdown Items
80% EtOH Prep Date
Date
Step File Placeholders
Log - Automatically attached
Sample Table
Sample Display Default = Expand
Well Sort Order = Row
Table Columns - Global Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in