TruSight Tumor 170 v2.0
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Last updated
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TruSight Tumor 170 v2.0 includes the following functionality:
Preconfigured TruSight Tumor 170 v2.0 protocol that supports the preparation of samples for the detection of gene variants across 170 gene targets in nucleic acids extracted from FFPE cancer tissue samples.
Automated calculation of sample and buffer volumes.
Automated calculation or display of reagents at every step in the protocol.
Automatic step transition when required.
Automatic placement of samples (when necessary).
Automated assignment of QC Pass/Fail, based on user-selected threshold values.
Routing script that allows sequencing of libraries using any Illumina sequencing instrument.
Protocol Type = Sample Prep
Next Steps Configuration
Master Step Name = DNA/RNA FFPE Extraction (TruSight Tumor 170 v2.0.10)
Step Type = Standard
Derived Sample Generation = Fixed, 2
Naming Convention = {LIST:{SubmittedSampleName}_DNA,{SubmittedSampleName}_RNA}
Reagent Kits
QIAGEN AllPrep DNA/RNA FFPE Kit
Supplier = Qiagen
Catalog Number = 80234
The version of DNA/RNA FFPE Extraction master step name may be different depending on the version of IPP installed.
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Sample Table
Column Headers
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Expanded View Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
LIMS ID (Container)
Built-in
Project
Project Name
Built-in
Step Data (Master Step Fields)
Field Name
Field Type
Options
Additional Options and Dropdown Items
Comment
Multiline Text
Step File Placeholders
Log File - Automatically attached
Sample Table
Sample Display Default = Expand
Well Sort Order = Column
Table Columns - Global Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Sample Type
Text Dropdown
Required Field
Custom Entries
Presets
DNA
RNA
Project
Project Name
Built-in
Protocol Type = Library Prep
Next Steps Configuration
Master Step Name = Qubit RNA QC (TruSight Tumor 170 v2.0.10)
Step Type = Standard QC
Measurement Generation = Fixed, 1
Naming Convention = {SubmittedSampleName}
The version of Qubit RNA QC master step name may be different depending on the version of IPP installed.
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Sample Table
Column Headers
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Sample Type
Text Dropdown
Required Field
Custom Entries
Presets
DNA
RNA
Derived Sample
Waiting
Built-in
Expanded View Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
LIMS ID (Container)
Built-in
Project
Project Name
Built-in
Step Data (Master Step Fields)
Field Name
Field Type
Options
Additional Options and Dropdown Items
Criteria 1 - Operator
Text Dropdown
Custom Entries
Presets
>=
<=
=
!=
Default = >=
Criteria 1 - Source Data Field
Text
Default = Concentration (ng/ul)
Criteria 1 - Threshold Value
Numeric
Default = 4.7
Decimal Places Displayed = 2
Criteria 2 - Operator
Text Dropdown
Custom Entries
Presets
>=
<=
=
!=
Default = >=
Criteria 2 - Source Data Field
Text
Default = Concentration (ng/ul)
Criteria 2 - Threshold Value
Numeric
Decimal Places Displayed = 2
Step File Placeholders
Log File - Automatically attached
Qubit Result File - Manually uploaded
Sample Table
Enable QC Flags = Yes
Sample Display Default = Expand
Well Sort Order = Column
File Column Options
File Column Display = Hide
File Attachment Method = Auto
Table Columns - Global Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Concentration (ng/ul)
Numeric
Decimal Places Displayed = 2
Derived Sample
Sample Name
Built-in
Measurement
Concentration (ng/ul)
Numeric
Decimal Places Displayed = 2
Project
Project Name
Built-in
Master Step Name = Denature and Anneal RNA (TruSight Tumor 170 v2.0.10)
Step Type = Standard
Derived Sample Generation = Fixed, 1
Naming Convention = {InputItemName}
Reagent Kits
TruSight Tumor 170, Box 1
Supplier = Illumina
Catalog Number = OP-101-1004
The version of Denature and Anneal RNA master step name may be different depending on the version of IPP installed.
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Sample Table
Column Headers
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
Well
Built-in
Derived Sample
Concentration (ng/ul)
Numeric
Decimal Places Displayed = 2
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Expanded View Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
LIMS ID (Container)
Built-in
Project
Project Name
Built-in
Step Data (Master Step Fields)
Field Name
Field Type
Options
Additional Options and Dropdown Items
Comment
Multiline Text
Dilution Concentration (ng/ul)
Numeric
Required Field
Range = 4.7 To 10
Decimal Places Displayed = 1
FSM (ul)
Text Dropdown
Custom Entries
Presets
27
72
144
216
RVT (ul)
Text Dropdown
Custom Entries
Presets
3
8
16
24
Thermal Cycler Program
Text Dropdown
Custom Entries
Presets
LQ-RNA
HQ-RNA
Step File Placeholders
Calculation Log File - Automatically attached
NextStep Log File - Automatically attached
Sample Table
Sample Display Default = Expand
Well Sort Order = Column
Table Columns - Global Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Buffer Volume (ul)
Numeric
Decimal Places Displayed = 2
Derived Sample
Sample Name
Built-in
Derived Sample
Sample Volume (ul)
Numeric
Decimal Places Displayed = 2
Project
Project Name
Built-in
Master Step Name = First Strand cDNA Synthesis v2.0
Step Type = No Outputs
The version of First Strand cDNA Synthesis master step name may be different depending on the version of IPP installed.
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Sample Table
Column Headers
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Expanded View Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
LIMS ID (Container)
Built-in
Project
Project Name
Built-in
Step Data (Master Step Fields)
Field Name
Field Type
Options
Additional Options and Dropdown Items
Comment
Multiline Text
Thermal Cycler Program
Text Dropdown
Custom Entries
Presets
Synthesize 1st Strand
1stSS
Step File Placeholders
Log File - Automatically attached
Sample Table
Sample Display Default = Collapse
Well Sort Order = Column
Table Columns - Global Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container