# DRAGEN Report

The **DRAGEN Report** is generated as part of the **DRAGEN miRNA Software** and provides key quality-control information for miRNA sequencing runs.\
The report is organized into the following sections:

* **Experimental Summary**
* **FastQC**
* **Mapping**

### Experimental Summary

The *Experimental Summary* tab contains two tables that describe the run configuration and a general overview of the data.

#### General Run Settings

| **Library Prep Kit**   | Illumina library preparation kit used to generate miRNA and other small RNA sequencing libraries (e.g., *miRNA Illumina Prep Kit*). |
| ---------------------- | ----------------------------------------------------------------------------------------------------------------------------------- |
| **Reference Database** | miRNA reference database version used for alignment and quantification (e.g., *miRBase\_v21*).                                      |
| **Software Version**   | Version of the DRAGEN miRNA analysis pipeline used to process the FASTQ files (e.g., *1.0.0*).                                      |
| **Species Used**       | Organism reference used for mapping input RNA (e.g., *Human*).                                                                      |

***

#### General Summary

<table data-header-hidden><thead><tr><th valign="top"></th><th valign="top"></th></tr></thead><tbody><tr><td valign="top">Sample</td><td valign="top"><br></td></tr><tr><td valign="top">Input Reads</td><td valign="top">Total input reads</td></tr><tr><td valign="top">Mapped Reads</td><td valign="top">Total mapped reads</td></tr><tr><td valign="top">% Mapped</td><td valign="top">Percentage of mapped reads</td></tr><tr><td valign="top">Average Read Length</td><td valign="top">Estimated average read length per sample</td></tr><tr><td valign="top">Average @ Score</td><td valign="top">Estimated average Q score per sample</td></tr><tr><td valign="top">Average % GC Content</td><td valign="top">Estimated Average %GC content per sample</td></tr></tbody></table>

### FastQC

This tab displays the following graphs:

* ﻿﻿Read Quality Distribution
* ﻿﻿%GC Content
* ﻿﻿Ambiguous Base Content by Position

### Mapping

This tab displays the following graph and tables:

• Characterized Reads

This graph contains the percentage of total reads classified to a given small RNA type (e.g., miRNA, hairpin, RNA, tRNA, piRNA, mRNA, other RNA reads). The missing percentage is attributed to unmapped reads.

#### Mapping Summary

<table data-header-hidden><thead><tr><th valign="top"></th><th valign="top"></th></tr></thead><tbody><tr><td valign="top">Index</td><td valign="top">Sample index</td></tr><tr><td valign="top">Sample</td><td valign="top">Sample ID defined by fasta names</td></tr><tr><td valign="top">Input Reads</td><td valign="top">Total reads input both mapped and unmapped</td></tr><tr><td valign="top">Mapped Reads</td><td valign="top">Number of mapped reads</td></tr><tr><td valign="top">% Mapped</td><td valign="top">Percentage of mapped reads from total reads</td></tr><tr><td valign="top">Unmapped Reads</td><td valign="top">Number of unmapped reads</td></tr><tr><td valign="top">% Unmapped</td><td valign="top">Percentage of unmapped reads from total reads</td></tr></tbody></table>

#### Mapped Reads

<table data-header-hidden><thead><tr><th valign="top"></th><th valign="top"></th></tr></thead><tbody><tr><td valign="top">Index</td><td valign="top">Sample Index</td></tr><tr><td valign="top">Sample</td><td valign="top">Sample ID defined by fasta names</td></tr><tr><td valign="top">miRNA</td><td valign="top">Number of reads mapped to micro RNAS</td></tr><tr><td valign="top">rRNA</td><td valign="top">Number of reads mapped to ribosomal RNA</td></tr><tr><td valign="top">tRNA</td><td valign="top">Number of reads mapped to transfer RNA</td></tr><tr><td valign="top">mRNA</td><td valign="top"><p>Number of reads mapped to messenger</p><p>RNA</p></td></tr><tr><td valign="top">piRNA</td><td valign="top">Number of reads mapped to piwi-interacting RNA</td></tr><tr><td valign="top">Hairpin</td><td valign="top">Number of reads mapped to hairpin</td></tr><tr><td valign="top">Other RNA</td><td valign="top">Number of reads mapped to other RNA types than the ones described above</td></tr></tbody></table>

#### Unmapped Reads

<table data-header-hidden><thead><tr><th valign="top"></th><th valign="top"></th></tr></thead><tbody><tr><td valign="top">Index</td><td valign="top">Sample Index</td></tr><tr><td valign="top">Sample</td><td valign="top">Sample ID</td></tr><tr><td valign="top">Too Short</td><td valign="top"><p>Reads with a length shorter than 16 nucleotides after adapter trimming with</p><p>Cutadapt</p></td></tr><tr><td valign="top">No Adapter</td><td valign="top"><p>Reads containing no adapter sequence</p><p>(AACTGTAGGCACCATCAAT)</p></td></tr><tr><td valign="top">UMI defective</td><td valign="top">Reads lacking a unique molecular identifier are defective or missing</td></tr><tr><td valign="top">Uncharacterized Mappable</td><td valign="top">During multiple alignment processes, reads are assigned their corresponding RNA identities. Uncharacterized mappable reads refer to those not classified as a specific RNA type but can be aligned to the reference genome</td></tr><tr><td valign="top">Uncharacterized Unmappable</td><td valign="top">Uncharacterized and unmappable RNA refers to RNA sequences that cannot be assigned to a specific RNA category or aligned with a reference genome</td></tr></tbody></table>
