DRAGEN Report

The DRAGEN Report is generated as part of the DRAGEN miRNA Software and provides key quality-control information for miRNA sequencing runs. The report is organized into the following sections:

• Experimental Summary

• FastQC

• Mapping

Experimental Summary

The Experimental Summary tab contains two tables that describe the run configuration and a general overview of the data.

General Run Settings

Library Prep Kit	Illumina library preparation kit used to generate miRNA and other small RNA sequencing libraries (e.g., miRNA Illumina Prep Kit).
Reference Database	miRNA reference database version used for alignment and quantification (e.g., miRBase_v21).
Software Version	Version of the DRAGEN miRNA analysis pipeline used to process the FASTQ files (e.g., 1.0.0).
Species Used	Organism reference used for mapping input RNA (e.g., Human).

---

General Summary

Sample
Input Reads	Total input reads
Mapped Reads	Total mapped reads
% Mapped	Percentage of mapped reads
Average Read Length	Estimated average read length per sample
Average @ Score	Estimated average Q score per sample
Average % GC Content	Estimated Average %GC content per sample

FastQC

This tab displays the following graphs:

• Read Quality Distribution

• %GC Content

• Ambiguous Base Content by Position

Mapping

This tab displays the following graph and tables:

• Characterized Reads

This graph contains the percentage of total reads classified to a given small RNA type (e.g., miRNA, hairpin, RNA, tRNA, piRNA, mRNA, other RNA reads). The missing percentage is attributed to unmapped reads.

Mapping Summary

Index	Sample index
Sample	Sample ID defined by fasta names
Input Reads	Total reads input both mapped and unmapped
Mapped Reads	Number of mapped reads
% Mapped	Percentage of mapped reads from total reads
Unmapped Reads	Number of unmapped reads
% Unmapped	Percentage of unmapped reads from total reads

Mapped Reads

Index	Sample Index
Sample	Sample ID defined by fasta names
miRNA	Number of reads mapped to micro RNAS
rRNA	Number of reads mapped to ribosomal RNA
tRNA	Number of reads mapped to transfer RNA
mRNA	Number of reads mapped to messenger RNA
piRNA	Number of reads mapped to piwi-interacting RNA
Hairpin	Number of reads mapped to hairpin
Other RNA	Number of reads mapped to other RNA types than the ones described above

Unmapped Reads

Index	Sample Index
Sample	Sample ID
Too Short	Reads with a length shorter than 16 nucleotides after adapter trimming with Cutadapt
No Adapter	Reads containing no adapter sequence (AACTGTAGGCACCATCAAT)
UMI defective	Reads lacking a unique molecular identifier are defective or missing
Uncharacterized Mappable	During multiple alignment processes, reads are assigned their corresponding RNA identities. Uncharacterized mappable reads refer to those not classified as a specific RNA type but can be aligned to the reference genome
Uncharacterized Unmappable	Uncharacterized and unmappable RNA refers to RNA sequences that cannot be assigned to a specific RNA category or aligned with a reference genome