MiSeq v8.2.0

The MiSeq Integration Package v8.2.0 only supports Clarity LIMS v6.2.

The Illumina MiSeq Integration v8.2.0 includes the following features:

• Preconfigured MiSeq Sequencing v3.2 workflow that maps to lab protocol steps and instrument runs.

• Automated sample sheet generation for use with MiSeq Control Software (MCS) and Local Run Manager.

• The following supported Local Run Manager analysis modules:

  • Generate FASTQ

  • Library QC

  • Resequencing

  • DNA Enrichment

  • DNA Amplicon

• Automated tracking of the sequencing run and the parsing of the following run statistics to Clarity LIMS:

  • Sequencing run metrics

  • Sequencing run parameters

  • Real-Time Analysis RTA v1 run directory location (and other run specific information)

• Preconfigured Library Prep Validation workflow that is used for validation purposes only. This workflow contains a single step protocol that models the library prep required to produce normalized libraries that are ready for the MiSeq Sequencing v3.2 workflow. MiSeq Integration Package v8.2.0 works with Library Prep Validation v2.3.1, which is installed with Illumina Preset Protocols (IPP) v2.6. For details, refer to MiSeq Integration v8.2.0 User Interaction, Validation and Troubleshooting

MiSeq Integration v8.2.0 runs as independent service and can be on the same server as other Clarity LIMS integrations. The integration is only required for MCS v4.0. If you are running MCS v3.0 or below, do not upgrade to this integration.