Demo Data

For more information about this product, check the Demo Data tab on the BSSH website. You can import a sequencing run, containing raw sequencing data, or a project with secondary analysis results.

The DRAGEN miRNA Demo Project includes samples prepared using the Illumina miRNA sequencing library preparation protocol and sequenced on MiSeq i100.

Experimental Details

The DRAGEN miRNA Demo Data is composed of replicates of human brain total RNA (HBTR), human liver total RNA (HLTR), and total RNA isolated from human whole blood were prepared using the Illumina miRNA prep kit at 100ng input. The libraries were sequenced on MiSeq i100 with 100M flowcell using a single read at 72bp read length with dual indexing. The samples were analyzed with the Basespace/ICA Dragen miRNA app.

Tertiary Analysis

After successfully generating data with Illumina Sequencers and completing secondary analysis with the DRAGEN miRNA app, you can gain further insights through the Illumina Connected Multiomics.

In the following session, there will be instructions on how to analyze the miRNA data further, going from the miRNA counts matrix to differential expression analysis. To do so, users shall input information about their data through a metadata file.

Creating a Metadata file

The metadata file, is a .tsv or .csv file that shall contain the following mandatory column:

• "SampleID": containing the sample names as written in the count matrix file

Users can then add extra columns that describes samples such as "Phenotype", "Sex", or "Status".

The following table illustrates the metadata format:

SampleID	Status	Sex
SampleA	Disease	Female
SampleB	Disease	Male
SampleC	Control	Female