Additional Sample Sheet Fields for Autolaunch

Autolaunch-compatible sample sheets contain the following fields specific to autolaunch configuration. For more information on DRAGEN TSO 500 sample sheet requirements, refer to DRAGEN TruSight Oncology 500 Analysis Software v2.5.2 Product Documentation

Parameter	Details	Required
Cloud_Data
Sample_ID	The unique ID to identify a sample. Must match a Sample_ID used in the TSO500S_Data section.	Yes
Sample_Type	Enter DNA or RNA. For HRD samples, this parameter must be DNA.	Yes
Pair_ID	A unique ID that links DNA and RNA from the same biological sample from the same individual. PairID shares, at most, one DNA and one RNA sample per run. eg, if a Sample_ID is TestSample1 DNA for DNA and TestSample1 RNA for RNA, the Pair_ID TestSample1 will link these samples that are on different rows in the sample sheet together. If the pair ID is associated with more than one DNA or RNA sample, the analysis fails.	Yes
Sample_Feature	For DNA samples that have undergone HRD enrichment, enter HRD in this column of the sample sheet. If the sample has not undergone HRD enrichment, leave the field empty.	Only required for HRD-enriched samples.
Sample_Description	Must meet the following requirements: - 1-50 characters. - Alpha numeric characters with underscores, dashes and spaces. If you enter a underscore, dash, or space, enter an alphanumeric character before and after. eg, Solid-FFPE_213.	No
Cloud_TSO500S_Settings
SoftwareVersion	The TSO500S software version	No
StartsFromFastq	Set the value to TRUE or FALSE. If autolaunching from BCL files, this must be set to FALSE.	Yes
Cloud_Data
Sample_ID	The same sample ID used in the Cloud_TSO500S_Data section.	No
ProjectName	The BaseSpace project name.	No
LibraryName	Combination of sample ID and index values in the following format: sampleID_Index_Index2	No
LibraryPrepKitName	The Library Prep Kit used.	No
IndexAdapterKitName	The Index Adapter Kit used.	No
Cloud_Settings
GeneratedVersion	The cloud GSS version used to create the sample sheet. Optional if manually updating a sample sheet.	No
CloudWorkflow	Ica_workflow_1	No
Cloud_TSO500_Pipeline	This value is a universal record number (URN . The valid values are: Solid—urn:ilmn:ica:pipeline:e8eff7ef-1683-4f63-a0ba9af542cd39e0#DRAGEN_TSO500_RUO_TISSUE_HT_v2_5_2_1_Pipeline Solid HRD —urn:ilmn:ica:pipeline:172270e9-3678-45a9-a9f4c9c7a0a32bb8#DRAGEN_TSO500_RUO_TISSUE_HRD_v2_5_2_1_Pipeline	Yes
BCLConvert_Pipeline	The value is a URN in the following format: urn:ilmn:ica:pipeline: <pipeline-ID>#<pipeline-name>	Yes