Run DRAGEN TruSight Oncology 500 Analysis Software

Start the DRAGEN TruSight Oncology 500 Analysis Software with the DRAGEN_TSO500.sh Bash script. The script is installed in the /usr/local/bin directory. The Bash script is executed on the command line and runs the software with Docker.

For arguments, refer to ADD LINK Command-Line Options. You can start from BCL files or from the FASTQ folder produced by BCL Convert. The following requirements apply for both methods:

• Path to the sequencing run or FASTQ folder. Copy the run or FASTQ folder to the DRAGEN server into the staging folder with the following recommended organization: /staging/runs/{RunID}. You can copy the run folder onto the DRAGEN server using Linux commands such as rsync. The sample sheet within the run folder is used unless otherwise specified through the command line.

• Run folder must be intact. Refer to Add Link Starting From BCL Files for input requirements.

• If the analysis output folder path is different from the default, provide the analysis output folder path. Refer to ADD LINK Command-Line Options.