Compatibility

The TruSight Oncology 500 support pages on the Illumina support site provide information on compatibility with Illumina sequencing systems.

Use BCL Convert to produce FASTQ files for DRAGEN TruSight Oncology 500 Analysis Software. Using bcl2fastq does not produce the same results and is discouraged.

Installation Requirements

DRAGEN TruSight Oncology 500 Analysis Software is compatible with Illumina DRAGEN Server v3 or v4.

Hardware

• DRAGEN server

• If performing TruSight Oncology 500 High-Throughput analysis, mkfifo is enabled on the network-attached storage (NAS .

Software

• By default Linux CentOS 7.9 operating system (or later) or Oracle Linux 8 (or later), is provided.

Storage Requirements

For optimal performance, run analysis on data stored locally on the DRAGEN server. Analysis of data stored on NAS can take longer and performance can be less reliable. The DRAGEN server provides an NVMe SSD in the /staging directory to use as the software output directory. Network-attached storage is required for long-term storage. When running the DRAGEN TruSight Oncology 500 Analysis Software, use the default settings or set the -analysisFolder command line option to a directory in /staging to make sure the DRAGEN server processes read and write data on the NVMe SSD. Before beginning analysis, develop a strategy to copy data from the DRAGEN server to a network‑attached storage. Delete output data on the DRAGEN server as soon as possible.

The following are the run and analysis output sizes for each sequencing system per 101 bp:

Sequencing System	Run Folder Output (Gb)	Analysis Output (Gb)	Minimum Disk Space (Gb)
NextSeq 500/550 and 550Dx (RUO) HO flow cell	32-55	82-85	150
NovaSeq 6000/Dx (RUO) SP Flow Cell	85-100	250-374	300
NovaSeq 6000/Dx (RUO) S1 Flow Cell	164-200	360-665	800
NovaSeq 6000/Dx (RUO) S2 Flow Cell	290-460	890-1600	1500
NovaSeq 6000/Dx (RUO) S4 Flow Cell	800-1200	2700-4100	3000

When launching an analysis, the software checks that the minimum disk space required is available. If the minimum disk space is not available, the software shows an error message and prevents analysis from starting. If disk space is exhausted during a run, the run shows an error and stops analyzing.

Permissions

Permissions Illumina recommends the following permissions for installing and running software:

• Non-root users must be a member of the Docker group to run Docker

• For more information on Docker permission requirements and alternatives to running as root, refer to the Docker documentation available on the Docker website

• Installing and uninstalling the DRAGEN TruSight Oncology 500 Analysis Software and running the system check requires root privileges

• Run DRAGEN TruSight Oncology 500 Analysis Software without being logged in as a root user. Running the DRAGEN TruSight Oncology 500 Analysis Software as root is not required or recommended.