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DRAGEN Single Cell RNA
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DRAGEN Single Cell RNA
  • Introduction
  • Get Started
    • Prerequisites
  • Run Set Up
    • Sample Sheet Introduction
    • Run Planning in BaseSpace Sequence Hub
    • Sample Sheet Requirements
    • Manual Launch on BaseSpace
  • Analysis Methods
    • FASTQ Processing
    • Error Correction
    • Transcript Counting
    • PIPseq CRISPR Mode
  • Analysis Results
    • Accessing Results
    • DRAGEN Report
      • Single-Cell RNA
      • Single-Cell Clustering
      • Trimmer
      • DRAGEN FastQC
      • Mapping
    • Secondary Analysis Results
  • Tertiary Analysis
    • Illumina Connected Multiomics
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Introduction

The DRAGEN Single Cell RNA application is a secondary analysis tool that can process multiplexed single cell RNA-Seq data in binary base call (BCL) files produced by NovaSeq 6000/6000Dx, NextSeq 1000/2000, and NovaSeq X Series sequencing systems to a cell-by-gene expression matrix.

You can perform secondary analysis in the cloud via BaseSpace Sequence Hub or Illumina Connected Analytics (ICA). When performing secondary analysis in the cloud, the analysis application launches automatically in BaseSpace Sequence Hub or ICA after the sequencing workflow completes.

NextPrerequisites

Last updated 4 months ago

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