NextSeq 1000/2000 On-Prem v1.0.0
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Clarity LIMS Software
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The NextSeq 1000/2000 On-Prem Integration Package v1.0.0 enables run planning through the v2 sample sheet that is generated or imported. The integration also tracks both sequencing run and GenerateFASTQ analysis run events without a connection to BaseSpace Sequence Hub or ICA.
The integration includes:
The preconfigured NextSeq 1000/2000 On-Prem Sequencing v1.0 workflow that maps to lab protocols, instrument runs, and GenerateFASTQ analysis.
The following preconfigured steps:
Library Pooling and Dilution (NextSeq 1000/2000 On-Prem Sequencing v1.0)
Load To Reagent Cartridge (NextSeq 1000/2000 On-Prem Sequencing v1.0)
AUTOMATED - Sequencing Run (NextSeq 1000/2000 On-Prem Sequencing v1.0)
Demultiplexing (NextSeq 1000/2000 On-Prem Sequencing v1.0)
Automated generation of the v2 sample sheet using Driver File Generator to set up Local Run Mode sequencing and GenerateFASTQ analysis runs.
Automated copying of the generated v2 sample sheet to a network drive location so that the instrument can import it.
Automated tracking of the sequencing run and parsing of run statistics into Clarity LIMS, including:
Progress and metrics of sequencing run
Sequencing run parameters
Reagent lot information
Sequencing run data directory location
Instrument software version
Real-Time Analysis (RTA) version and other run specific information
Automated tracking of local secondary analysis using DRAGEN (up to BCL Convert only) and parsing of demultiplexing metrics into Clarity LIMS. The local BCL Convert is supported up to v4.2.7.
[Optional] Preconfigured Library Prep Validation v2.3.3 protocol used for validation purposes only. This workflow contains a single-step protocol that models the library prep required to produce libraries tagged with index sequences that are ready for the NextSeq 1000/2000 On-Prem Sequencing v1.0 workflow. For more information, refer to .