# NextSeq 1000/2000 On-Prem v1.0.0

The NextSeq 1000/2000 On-Prem Integration Package v1.0.0 enables run planning through the v2 sample sheet that is generated or imported. The integration also tracks both sequencing run and GenerateFASTQ analysis run events without a connection to BaseSpace Sequence Hub or ICA.

The integration includes:

* The preconfigured NextSeq 1000/2000 On-Prem Sequencing v1.0 workflow that maps to lab protocols, instrument runs, and GenerateFASTQ analysis.
* The following preconfigured steps:
  * Library Pooling and Dilution (NextSeq 1000/2000 On-Prem Sequencing v1.0)
  * Load To Reagent Cartridge (NextSeq 1000/2000 On-Prem Sequencing v1.0)
  * AUTOMATED - Sequencing Run (NextSeq 1000/2000 On-Prem Sequencing v1.0)
  * Demultiplexing (NextSeq 1000/2000 On-Prem Sequencing v1.0)
* Automated generation of the v2 sample sheet using Driver File Generator to set up Local Run Mode sequencing and GenerateFASTQ analysis runs.
* Automated copying of the generated v2 sample sheet to a network drive location so that the instrument can import it.
* Automated tracking of the sequencing run and parsing of run statistics into Clarity LIMS, including:
  * Progress and metrics of sequencing run
  * Sequencing run parameters
  * Reagent lot information
  * Sequencing run data directory location
  * Instrument software version
  * Real-Time Analysis (RTA) version and other run specific information
* Automated tracking of local secondary analysis using DRAGEN (up to BCL Convert only) and parsing of demultiplexing metrics into Clarity LIMS. The local BCL Convert is supported up to v4.2.7.
* \[Optional] Preconfigured Library Prep Validation v2.3.3 protocol used for validation purposes only. This workflow contains a single-step protocol that models the library prep required to produce libraries tagged with index sequences that are ready for the NextSeq 1000/2000 On-Prem Sequencing v1.0 workflow. For more information, refer to [NextSeq 1000/2000 On-Prem Integration v1.0.0 User Interaction, Validation and Troubleshooting](https://help.connected.illumina.com/clarity-lims/instruments-and-integrations/nextseq1k2k-onprem/nextseq1k2k-onprem-v1.0.0/nextseq1k2k-onprem-v1.0.0-user-interaction-validation-and-troubleshooting).