iCredits
iCredits are used to purchase data storage and analysis within the Illumina cloud software ecosystem. One iCredit is equal to one US dollar (USD). iCredit balance and usage can be viewed in the Usage Explorer. All estimates were done in AWS US East (N. Virginia) region.
Default Workflows
Default workflows are out-of-the-box workflows included in Connected Multiomics Basic access. They include QC and dimension reduction features and are available for Illumina assays.
Default: Illumina Proteomics
Illumina Protein Prep 9.5K with 96 samples
0.01
0.96
Default: Illumina Single Cell Transcriptomics
Compute needs vary based on number of cells and reads obtained. Estimates below are for default analyses created for each sample in the study.
Illumina Single Cell 3’ RNA Prep T100 kit with 2 sample that has 318,910 cells and 40,371 features and 2.19 GB
2.6
5.3
Illumina Single Cell 3’ RNA Prep T20 kit with 4 samples with 99,570 cells, 40,563 features, and 587.7 MB
0.4
1.7
Illumina Single Cell 3’ RNA Prep T10 kit with 8 samples with 89,372 cells, 40,371 features and 513.4 MB
0.2
1.8
Default: Illumina Spatial Transcriptomics
DRAGEN secondary analysis provides tertiary calculations used as inputs to Connected Multiomics default workflow. If recalculation is needed, additional compute is required. See DRAGEN Secondary Analysis Compute and Storage for more detail about DRAGEN pipeline compute charges
Illumina spatial transcriptome technology with 1 sample that has 380k cells and approx 5B reads
0.01
0.01
Default: Illumina Bulk Transcriptomics
Compute needs vary based on number of features and reads obtained.
Illumina Stranded mRNA Prep with 61 samples that has 45,493 transcripts and 12 MB
<0.01
0.22
Illumina Stranded Prep with 8 samples that has 42,767 transcripts and 4.32 MB
<0.01
0.05
Illumina Stranded Total RNA Prep with 18 samples that has 39,466 transcripts and 5.41 MB
<0.01
0.07
Default: Illumina miRNA
Compute needs vary based on number of features and reads obtained.
Illumina miRNA with 6 samples with 821 features and ~102M reads
<0.01
<0.01
Illumina miRNA with 96 samples with 821 features
<0.01
<0.01
Default: Illumina 5-Base DNA
Varies based on sequencing depth and Germline versus Somatic differences
Methylome only (6 samples demo data)
0.69
4.14
Custom Workflows
Custom: Illumina Proteomics
Based on Tutorial Study Illumina Protein Prep (Custom).
Import
0.08
Filtered Counts
0
3 sec
Features in Gene Symbol List
0
3 sec
Normalized counts
0
1 sec
GSEA
0
18 sec
Limma-trend
0
20 sec
PCA
0
4 sec
Hierarchical clustering / heatmap
0
1 sec
Pathway enrichment
0.02
1 sec
Total iCredits
0.1
Custom: Illumina Single Cell Transcriptomics
Based on Tutorial Study Illumina Single Cell 3' Prep (Default).
Import
1.8
41 min 28 sec
Normalization
0.6
24 min 24 sec
PCA
5.4
97 min 42 sec
Graph-based clustering
0.8
10 min 55 sec
Compute biomarkers
2.9
68 min 23 sec
UMAP
0.01
1 min 37 sec
Total iCredits
9.72
Custom: Illumina Spatial Transcriptomics
Based on Tutorial Study Illumina Spatial Transcriptome.
Import
0
34 sec
Spatial report
0
0 sec
Filter observations
0.09
1 min 13 sec
Filter Features
1.33
24 min 45 sec
Normilization
0.48
29 min 6 sec
ScType cell classification
0.17
1 min 14 sec
BANKSY - Spatial Domain Identification
3.08
43 min 23 sec
ANOVA
1.06
9 min 31 sec
Differential analysis filter
0.46
6 min 32 sec
Correlation Engine pathway
0.13
22 min 27 sec
Total iCredits
6.80
Custom: Illumina Bulk Transcriptomics
Based on Tutorial Study Illumina Stranded Total RNA Prep (Custom).
Import
0.05
20 sec
Annotate features
< 0.01
1 sec
Normalization
< 0.01
2 sec
PCA
< 0.01
2 sec
Differential analysis
< 0.01
15 sec
Filtering differential expression results
< 0.01
3 sec
Gene set enrichment
< 0.01
4 sec
Pathway analysis
< 0.01
< 1 sec
Heatmap
0.02
16 sec
Bubble map
< 0.01
12 sec
Total iCredits
0.07
Custom: Illumina miRNA
Based on Tutorial Study (6 samples, 821 features).
Normalization
0
1 sec
PCA
0
3 sec
Hierarchical clustering
0
2 sec
Total iCredits
<0.01
Custom: Illumina 5-Base DNA
Based on Tutorial Study Illumina 5-base DNA. (6 samples, Methylome only)
Import 5-base methylation data into a custom analysis
3.99
41 min
5-base methylation QC report
0
0 sec
PCA
0.08
3.5 min
DSS
1.98
40 min
Filter DSS results
0
7 sec
Annotate regions
0
3 sec
Gene set, pathway enrichment
0
7 sec
Get Regional Methylation
0.06
2 min
Get Regional Methylation > PCA
0
1 sec
Get Regional Methylation > Annotate regions
0
2 sec
Total iCredits
6.11
DRAGEN Secondary Analysis Compute and Storage
DRAGEN Secondary Analysis Compute and StorageDRAGEN Protein Quantification
DRAGEN Protein Quantification is no charge for compute as these charges are included in the assay kit purchase.
Illumina Protein Prep 9.5K
0
<0.01
DRAGEN Single Cell RNA
DRAGEN Single Cell RNA is no charge for compute as these charges are included in the assay kit purchase. Estimated storage costs below include outputs from a DRAGEN Single Cell RNA analysis with the default options (no BAM output is generated by default).
Illumina Single Cell 3’ RNA Prep T100 kit with 2 sample that has 318,910 cells and 40,371 features and 2.19 GB
0
0.16
Illumina Single Cell 3’ RNA Prep T20 kit with 4 samples with 99,570 cells, 40,563 features, and 587.7 MB
0
0.08
Illumina Single Cell 3’ RNA Prep T10 kit with 8 samples with 89,372 cells, 40,371 features and 513.4 MB
0
.04
DRAGEN Spatial Transcriptome
Available to early access customers only.
Illumina Spatial Transcriptome slide with 4 mouse kidney samples (cell counts: 322k, 287k, 313k, and 277k), sequenced with one 25B
80
20
6.1
Illumina Spatial Transcriptome slide with 4 human breast samples (cell counts: 389k, 351k, 717k and 718k), sequenced with one 25B
146
37
7.2
DRAGEN RNA
Varies based on input, sequencing depth and functionality run.
NovaSeq X (10B): Illumina Stranded Total RNA with 18 samples (File size: 141.00 GB)
33.7
1.9
3.2
NovaSeq X (1.5B): Illumina Stranded mRNA prep with 61 samples (File size: 184.63 GB)
45.0
0.7
4.2
DRAGEN miRNA
DRAGEN miRNA is no charge for compute as these charges are included in the assay kit purchase.
Illumina miRNA with 96 samples with 821 features (File size: 18.47 GB)
0
0.4
Illumina miRNA with 48 samples with 821 features (File size: 9.24 GB)
0
0.2
DRAGEN 5-Base DNA Prep and 5-Base DNA Prep with Enrichment
Varies based on sequencing depth and functionality run.
Germline 5-Base Genome
(SNV + CNV + SV) at 35-40X
5
2.5
Methylome + Tumor-only Somatic VC at >100x
12
5
Targeted Somatic SNVs + Methylation (1MB panel) at > 100x
< 1
1
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