Biological Classification

The Biological Classification section of the Biomarker Details page summarizes all biological assertions previously created for the variant across all sources (i.e., My Knowledge Base, OncoKB, CKB).

If you have existing knowledge to upload to the system, refer to Upload Assertions.

You can filter assertions by source, diseases, and summary. Filter changes are saved and applied across variants within a case and can be reset if needed.

Assertion Details

Field Name	Description
Directional Arrows	Opens to view the assertion summary.
Update Date	The last date that the assertion was updated.
Biomarker	Indicates how the assertion is related to the variant (for example, specific amino acid change or specific exon change).
HRD	Indicates whether an assertion is associated with HRD positive or negative. This column is available for GIS and variants when the selected transcript is for BRCA1/2.
Classification	This column displays the classification as indicated by the knowledge base.
Type	The assertion type (for example, biological). For more information, refer to Interpret a Variant.
Disease	The disease associated with the classification.
Actions	This column contains the following available actions: • Add to report • Archive, if the assertion is in My Knowledge Base • Copy to New Assertion, if the assertion is not in My Knowledge Base • View past cases To edit an assertion, add it to the report before editing. For more information, refer to Manage Assertions. When reporting an assertion that is not from My Knowledge Base (e.g., OncoKB), classifications are reported with the following adjustments: Connected Insights OncoKB Oncogenic Oncogenic Likely Oncogenic Likely Oncogenic Uncertain Significance Inconclusive Likely Benign Likely Neutral Resistance Resistance
Connected Insights	OncoKB
Oncogenic	Oncogenic
Likely Oncogenic	Likely Oncogenic
Uncertain Significance	Inconclusive
Likely Benign	Likely Neutral
Resistance	Resistance

Available Knowledge Bases

Memorial Sloan Kettering OncoKB

Connected Insights provides the Memorial Sloan Kettering OncoKB. For more information, refer to the OncoKB website.

The following from OncoKB is not supported:

• Non-compliant HGVS

• Unspecified positions: Epigenetic Silencing, ARv567es, AR-V7, DNMT3B7, vIII, vII, vV, TGFBR1*6A, Overexpression, Wildtype, Promoter Hypermethylation, Hypermethylation, Kinase Domain Duplication, Internal Tandem Duplication, Partial Tandem Duplication

Genomenon Cancer Knowledge Base (CKB)

Connected Insights provides the Genomenon Cancer Knowledge Base (formerly Jackson Laboratory Clinical Knowledge Base). For more information, refer to the Genomenon CKB website.

The following from CKB is not supported:

• Complex molecular profiles (for example, co-occuring biomarker assertions)

• Emerging and risk factor evidence types

• Class #, over exp, dec exp, hypermethylation, hypomethylation, dup exonX, dup exonX-X, LOH, loss, negative (except for HRD andMSI), positive (except for HRD), and wild type variants.