Prerequisites

Before starting the installation or update of Connected Insights - Local software, make sure that the following prerequisites are met.

1. DRAGEN Server v4 Configuration

Ensure the DRAGEN Server v4 configuration is as below:

• DRAGEN server v4 with Oracle Linux 8. The preferred version of Oracle Linux 8 is preinstalled on the DRAGEN server v4. The DRAGEN server v4 software pipeline is also included.

• DRAGEN server v4 setup shall be completed following the Illumina DRAGEN Server v4 Installation Guide (document #200015717). For more information, refer to Illumina DRAGEN Server v4 Installation Guide on the Illumina support site.

• DRAGEN server v4 is idle during installation or update of Illumina Connected Insights - Local software.

If you are setting the hostname for a DRAGEN server v4, ensure the server has FQDN (Fully Qualified Domain Name) (Example: If the server's hostname is onpremtest and domain is abcd.com, then the server shall be registered as onpremtest.abcd.com in your organization's DNS(Domain Name Server)). If your organization does not have a DNS server, then ensure to setup the server with a static IP and the /etc/resolv.conf file shall be an empty file with no contents in it. Once set up, this configuration will be required for installation and cannot be changed throughout the software's usage. If it changes in the between, then application may not behave as intended and could possibly throw errors. Thus, ensure to finalize the configuration i.e. with FQDN (Or) with Static IP before starting the installation process mentioned in this document.

2. Root or sudoer privileges to access DRAGEN Server v4

You must have root or sudo privileges to run installation commands. To access the DRAGEN server v4 and run commands from the command-line interface, you should be familiar with the Linux commands used to install the software and config file (in case of upgrade from current installed version to the new version).

3. Mounted External Storage Drive with read/write access. It is highly recommended you create a user on the mounted storage which has read and write permission and not use a root user.

An external storage drive must be connected to the DRAGEN server v4 directly or through the network. If an external storage drive meeting the requirements below is not mounted already, refer to the How to section for details on how to mount an external storage drive.

Below are the minimum requirements of the external storage which shall be mounted on the DRAGEN server v4.

• Configured with CIFS/SMB or NFS protocols only. Recommended to use NFS v3.5 and above or CIFS/SMB v3.1 and above protocol for data security. Illumina also recommends an encrypted data storage drive to safeguard your data.

• 100 gigabytes (GB) of available storage space.

• Recommended: 10 Gb (Gigabits) per second of network speed.

The external storage drive is used by Connected Insights - Local software to read inputs and write outputs.

• For inputs, Connected Insights - Local software reads secondary analysis data (for example, VCF and BAM files).

• For outputs, Connected Insights - Local software writes analysis output data and backup files (for example, annotated VCF files, visualization files, and PDF report files).

4. Illumina provided package URLs are accessible in your network

In Illumina's welcome email or customer notification email, all the links to download the packages shall be accessible. If you are unable to reach to any of the sites, please check with your IT to whitelist the URLs. The example URLs are https://support.illumina.com/downloads/* and https://illumina-use1-ici-local-release-data.s3.amazonaws.com/releases/knowledgebases/*

5. A Personal Computer

A personal computer which has a remote access client tool to connect to the DRAGEN server v4 to run the commands and a browser to access Connected Insights - Local software application after software is installed.