NovaSeq X Sample Sheet Version History

• ✅ = Supported

• 🟨 = Supported. Change in definition from prior release. See Release Notes and/or Sample Sheet Definition for each version for details.

• Blank = Not supported

Pipeline	Section	Type	Key Word	4.1.5	4.1.7	4.1.23	4.3.13
DRAGEN BCL Convert	[BCLConvert_Settings]	Required	SoftwareVersion	✅	✅	✅	✅
DRAGEN BCL Convert	[BCLConvert_Settings]	Required	AppVersion	✅	✅	✅	✅
DRAGEN BCL Convert	[BCLConvert_Settings]	Required	FastqCompressionFormat	✅	✅	✅	✅
DRAGEN BCL Convert	[BCLConvert_Settings]	Optional	CreateFastqForIndexReads	✅	✅	✅	✅
DRAGEN BCL Convert	[BCLConvert_Settings]	Optional	TrimUMI	✅	✅	✅	✅
DRAGEN BCL Convert	[BCLConvert_Settings]	Import Option	AdapterBehavior				✅
DRAGEN BCL Convert	[BCLConvert_Settings]	Import Option	AdapterStringency				✅
DRAGEN BCL Convert	[BCLConvert_Settings]	Import Option	MinimumTrimmedReadLength				✅
DRAGEN BCL Convert	[BCLConvert_Settings]	Import Option	MinimumAdapterOverlap				✅
DRAGEN BCL Convert	[BCLConvert_Settings]	Import Option	MaskShortReads				✅
DRAGEN BCL Convert	[BCLConvert_Settings]	Import Option	NoLaneSplitting				✅
DRAGEN BCL Convert	[BCLConvert_Settings]	Import Option	FindAdaptersWithIndels				✅
DRAGEN BCL Convert	[BCLConvert_Settings]	Import Option	IndependentIndexCollisionCheck				✅
DRAGEN BCL Convert	[BCLConvert_Settings]	Import Option	GenerateFastqcMetrics				✅
DRAGEN BCL Convert	[BCLConvert_Data]	Required	SampleID	✅	✅	✅	✅
DRAGEN BCL Convert	[BCLConvert_Data]	Optional	Lane	✅	✅	✅	✅
DRAGEN BCL Convert	[BCLConvert_Data]	Optional	Index	✅	✅	✅	✅
DRAGEN BCL Convert	[BCLConvert_Data]	Optional	Index2	✅	✅	✅	✅
DRAGEN BCL Convert	[BCLConvert_Data]	Optional	OverrideCycles	✅	✅	✅	✅
DRAGEN BCL Convert	[BCLConvert_Data]	Optional	AdapterRead1	✅	✅	✅	✅
DRAGEN BCL Convert	[BCLConvert_Data]	Optional	AdapterRead2	✅	✅	✅	✅
DRAGEN BCL Convert	[BCLConvert_Data]	Optional	BarcodeMismatchesIndex1	✅	✅	✅	✅
DRAGEN BCL Convert	[BCLConvert_Data]	Optional	BarcodeMismatchesIndex1	✅	✅	✅	✅
DRAGEN BCL Convert	[BCLConvert_Data]	Optional	RGID			✅	✅
DRAGEN BCL Convert	[BCLConvert_Data]	Optional	RGPU			✅	✅
DRAGEN BCL Convert	[BCLConvert_Data]	Optional	RGPL			✅	✅
DRAGEN BCL Convert	[BCLConvert_Data]	Optional	RGLB			✅	✅
DRAGEN BCL Convert	[BCLConvert_Data]	Optional	RGCN			✅	✅
DRAGEN BCL Convert	[BCLConvert_Data]	Optional	RGPM			✅	✅
DRAGEN BCL Convert	[BCLConvert_Data]	Import Option	Sample_Project			✅	✅
DRAGEN Germline	[DragenGermline_Settings]	Required	SoftwareVersion	✅	✅	✅	✅
DRAGEN Germline	[DragenGermline_Settings]	Required	AppVersion	✅	✅	✅	✅
DRAGEN Germline	[DragenGermline_Settings]	Required	KeepFastq	✅	✅	✅	✅
DRAGEN Germline	[DragenGermline_Settings]	Required	MapAlignOutFormat	✅	✅	✅	✅
DRAGEN Germline	[DragenGermline_Data]	Required	SampleID	✅	✅	✅	✅
DRAGEN Germline	[DragenGermline_Data]	Required	ReferenceGenomeDir	✅	✅	✅	✅
DRAGEN Germline	[DragenGermline_Data]	Required	VariantCallingMode	✅	🟨	🟨	🟨
DRAGEN Germline	[DragenGermline_Data]	Required	QcCoverage1BedFile			✅	✅
DRAGEN Germline	[DragenGermline_Data]	Required	QcCoverage2BedFile			✅	✅
DRAGEN Germline	[DragenGermline_Data]	Required	QcCoverage3BedFile			✅	✅
DRAGEN Germline	[DragenGermline_Data]	Required	QcCrossContaminationVcfFile			✅	✅
DRAGEN Enrichment	[DragenEnrichment_Settings]	Required	SoftwareVersion	✅	✅	✅	✅
DRAGEN Enrichment	[DragenEnrichment_Settings]	Required	AppVersion	✅	✅	✅	✅
DRAGEN Enrichment	[DragenEnrichment_Settings]	Required	KeepFastq	✅	✅	✅	✅
DRAGEN Enrichment	[DragenEnrichment_Settings]	Optional	MapAlignOutFormat	✅	✅	✅	✅
DRAGEN Enrichment	[DragenEnrichment_Data]	Required	SampleID	✅	✅	✅	✅
DRAGEN Enrichment	[DragenEnrichment_Data]	Required	ReferenceGenomeDir	✅	✅	✅	✅
DRAGEN Enrichment	[DragenEnrichment_Data]	Conditionally Required	BedFile	✅	✅	✅	✅
DRAGEN Enrichment	[DragenEnrichment_Data]	Required	GermlineOrSomatic	✅	✅	✅	✅
DRAGEN Enrichment	[DragenEnrichment_Data]	Optional	AuxNoiseBaselineFile	✅	✅	✅	✅
DRAGEN Enrichment	[DragenEnrichment_Data]	Optional	AuxCnvPanelOfNormalsFile	✅	✅	✅	✅
DRAGEN Enrichment	[DragenEnrichment_Data]	Required	VariantCallingMode	✅	✅	✅	🟨
DRAGEN Enrichment	[DragenEnrichment_Data]	Optional	AuxCnvPopBAlleleVcfFile				✅
DRAGEN Enrichment	[DragenEnrichment_Data]	Optional	AuxGermlineTaggingFile				✅
DRAGEN RNA	[DragenRna_Settings]	Required	SoftwareVersion	✅	✅	✅	✅
DRAGEN RNA	[DragenRna_Settings]	Required	AppVersion	✅	✅	✅	✅
DRAGEN RNA	[DragenRna_Settings]	Required	MapAlignOutFormat	✅	✅	✅	✅
DRAGEN RNA	[DragenRna_Settings]	Required	KeepFastq	✅	✅	✅	✅
DRAGEN RNA	[DragenRna_Settings]	Optional	DifferentialExpressionEnable	✅	✅	✅	✅
DRAGEN RNA	[DragenRna_Data]	Required	SampleID	✅	✅	✅	✅
DRAGEN RNA	[DragenRna_Data]	Required	ReferenceGenomeDir	✅	✅	✅	✅
DRAGEN RNA	[DragenRna_Data]	Optional	RnaGeneAnnotationFile	✅	✅	✅	✅
DRAGEN RNA	[DragenRna_Data]	Required	RnaPipelineMode	✅	✅	✅	✅
DRAGEN RNA	[DragenRna_Data]	Optional	DownSampleNumReads	✅	✅	✅	✅
DRAGEN RNA	[DragenRna_Data]	Optional	Comparison1	✅	✅	✅	✅
DRAGEN RNA	[DragenRna_Data]	Optional	Comparison2	✅	✅	✅	✅
DRAGEN RNA	[DragenRna_Data]	Optional	Comparison3	✅	✅	✅	✅
DRAGEN RNA	[DragenRna_Data]	Optional	Comparison4	✅	✅	✅	✅
DRAGEN RNA	[DragenRna_Data]	Optional	Comparison5	✅	✅	✅	✅
DRAGEN Somatic	[DragenSomatic_Settings]	Required	SoftwareVersion			✅	✅
DRAGEN Somatic	[DragenSomatic_Settings]	Required	AppVersion			✅	✅
DRAGEN Somatic	[DragenSomatic_Settings]	Required	MapAlignOutFormat			✅	✅
DRAGEN Somatic	[DragenSomatic_Settings]	Required	KeepFastq			✅	✅
DRAGEN Somatic	[DragenSomatic_Data]	Optional	AuxNoiseBaselineFile			✅	✅
DRAGEN Somatic	[DragenSomatic_Data]	Optional	AuxSvNoiseBaselineFile			✅	✅
DRAGEN Somatic	[DragenSomatic_Data]	Optional	AuxCnvPopBAlleleVcfFile			✅	✅
DRAGEN Somatic	[DragenSomatic_Data]	Optional	AuxGermlineTaggingFile			✅	✅
DRAGEN Somatic	[DragenSomatic_Data]	Required	VariantCallingMode			✅	✅
DRAGEN Somatic	[DragenSomatic_Data]	Required	Sample_ID			✅	✅
DRAGEN Methylation	[DragenMethylation_Settings]	Required	SoftwareVersion			✅	✅
DRAGEN Methylation	[DragenMethylation_Settings]	Required	AppVersion			✅	✅
DRAGEN Methylation	[DragenMethylation_Settings]	Required	MapAlignOutFormat			✅	✅
DRAGEN Methylation	[DragenMethylation_Settings]	Required	KeepFastq			✅	✅
DRAGEN Methylation	[DragenMethylation_Settings]	Required	UsesTaps			✅	✅
DRAGEN Methylation	[DragenMethylation_Data]	Required	ReferenceGenomeDir			✅	✅
DRAGEN Methylation	[DragenMethylation_Data]	Required	MethylationProtocol			✅	✅
DRAGEN Methylation	[DragenMethylation_Data]	Required	Sample_ID			✅	✅