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NovaSeq X Sample Sheet Version History

  • ✅ = Supported

  • 🟨 = Supported. Change in definition from prior release. See Release Notes and/or Sample Sheet Definition for each version for details.

  • Blank = Not supported

Pipeline
Section
Type
Key Word
4.1.5
4.1.7
4.1.23
4.3.13

DRAGEN BCL Convert

[BCLConvert_Settings]

Required

SoftwareVersion

✅

✅

✅

✅

DRAGEN BCL Convert

[BCLConvert_Settings]

Required

AppVersion

✅

✅

✅

✅

DRAGEN BCL Convert

[BCLConvert_Settings]

Required

FastqCompressionFormat

✅

✅

✅

✅

DRAGEN BCL Convert

[BCLConvert_Settings]

Optional

CreateFastqForIndexReads

✅

✅

✅

✅

DRAGEN BCL Convert

[BCLConvert_Settings]

Optional

TrimUMI

✅

✅

✅

✅

DRAGEN BCL Convert

[BCLConvert_Settings]

Import Option

AdapterBehavior

✅

DRAGEN BCL Convert

[BCLConvert_Settings]

Import Option

AdapterStringency

✅

DRAGEN BCL Convert

[BCLConvert_Settings]

Import Option

MinimumTrimmedReadLength

✅

DRAGEN BCL Convert

[BCLConvert_Settings]

Import Option

MinimumAdapterOverlap

✅

DRAGEN BCL Convert

[BCLConvert_Settings]

Import Option

MaskShortReads

✅

DRAGEN BCL Convert

[BCLConvert_Settings]

Import Option

NoLaneSplitting

✅

DRAGEN BCL Convert

[BCLConvert_Settings]

Import Option

FindAdaptersWithIndels

✅

DRAGEN BCL Convert

[BCLConvert_Settings]

Import Option

IndependentIndexCollisionCheck

✅

DRAGEN BCL Convert

[BCLConvert_Settings]

Import Option

GenerateFastqcMetrics

✅

DRAGEN BCL Convert

[BCLConvert_Data]

Required

SampleID

✅

✅

✅

✅

DRAGEN BCL Convert

[BCLConvert_Data]

Optional

Lane

✅

✅

✅

✅

DRAGEN BCL Convert

[BCLConvert_Data]

Optional

Index

✅

✅

✅

✅

DRAGEN BCL Convert

[BCLConvert_Data]

Optional

Index2

✅

✅

✅

✅

DRAGEN BCL Convert

[BCLConvert_Data]

Optional

OverrideCycles

✅

✅

✅

✅

DRAGEN BCL Convert

[BCLConvert_Data]

Optional

AdapterRead1

✅

✅

✅

✅

DRAGEN BCL Convert

[BCLConvert_Data]

Optional

AdapterRead2

✅

✅

✅

✅

DRAGEN BCL Convert

[BCLConvert_Data]

Optional

BarcodeMismatchesIndex1

✅

✅

✅

✅

DRAGEN BCL Convert

[BCLConvert_Data]

Optional

BarcodeMismatchesIndex1

✅

✅

✅

✅

DRAGEN BCL Convert

[BCLConvert_Data]

Optional

RGID

✅

✅

DRAGEN BCL Convert

[BCLConvert_Data]

Optional

RGPU

✅

✅

DRAGEN BCL Convert

[BCLConvert_Data]

Optional

RGPL

✅

✅

DRAGEN BCL Convert

[BCLConvert_Data]

Optional

RGLB

✅

✅

DRAGEN BCL Convert

[BCLConvert_Data]

Optional

RGCN

✅

✅

DRAGEN BCL Convert

[BCLConvert_Data]

Optional

RGPM

✅

✅

DRAGEN BCL Convert

[BCLConvert_Data]

Import Option

Sample_Project

✅

✅

DRAGEN Germline

[DragenGermline_Settings]

Required

SoftwareVersion

✅

✅

✅

✅

DRAGEN Germline

[DragenGermline_Settings]

Required

AppVersion

✅

✅

✅

✅

DRAGEN Germline

[DragenGermline_Settings]

Required

KeepFastq

✅

✅

✅

✅

DRAGEN Germline

[DragenGermline_Settings]

Required

MapAlignOutFormat

✅

✅

✅

✅

DRAGEN Germline

[DragenGermline_Data]

Required

SampleID

✅

✅

✅

✅

DRAGEN Germline

[DragenGermline_Data]

Required

ReferenceGenomeDir

✅

✅

✅

✅

DRAGEN Germline

[DragenGermline_Data]

Required

VariantCallingMode

✅

🟨

🟨

🟨

DRAGEN Germline

[DragenGermline_Data]

Required

QcCoverage1BedFile

✅

✅

DRAGEN Germline

[DragenGermline_Data]

Required

QcCoverage2BedFile

✅

✅

DRAGEN Germline

[DragenGermline_Data]

Required

QcCoverage3BedFile

✅

✅

DRAGEN Germline

[DragenGermline_Data]

Required

QcCrossContaminationVcfFile

✅

✅

DRAGEN Enrichment

[DragenEnrichment_Settings]

Required

SoftwareVersion

✅

✅

✅

✅

DRAGEN Enrichment

[DragenEnrichment_Settings]

Required

AppVersion

✅

✅

✅

✅

DRAGEN Enrichment

[DragenEnrichment_Settings]

Required

KeepFastq

✅

✅

✅

✅

DRAGEN Enrichment

[DragenEnrichment_Settings]

Optional

MapAlignOutFormat

✅

✅

✅

✅

DRAGEN Enrichment

[DragenEnrichment_Data]

Required

SampleID

✅

✅

✅

✅

DRAGEN Enrichment

[DragenEnrichment_Data]

Required

ReferenceGenomeDir

✅

✅

✅

✅

DRAGEN Enrichment

[DragenEnrichment_Data]

Conditionally Required

BedFile

✅

✅

✅

✅

DRAGEN Enrichment

[DragenEnrichment_Data]

Required

GermlineOrSomatic

✅

✅

✅

✅

DRAGEN Enrichment

[DragenEnrichment_Data]

Optional

AuxNoiseBaselineFile

✅

✅

✅

✅

DRAGEN Enrichment

[DragenEnrichment_Data]

Optional

AuxCnvPanelOfNormalsFile

✅

✅

✅

✅

DRAGEN Enrichment

[DragenEnrichment_Data]

Required

VariantCallingMode

✅

✅

✅

🟨

DRAGEN Enrichment

[DragenEnrichment_Data]

Optional

AuxCnvPopBAlleleVcfFile

✅

DRAGEN Enrichment

[DragenEnrichment_Data]

Optional

AuxGermlineTaggingFile

✅

DRAGEN RNA

[DragenRna_Settings]

Required

SoftwareVersion

✅

✅

✅

✅

DRAGEN RNA

[DragenRna_Settings]

Required

AppVersion

✅

✅

✅

✅

DRAGEN RNA

[DragenRna_Settings]

Required

MapAlignOutFormat

✅

✅

✅

✅

DRAGEN RNA

[DragenRna_Settings]

Required

KeepFastq

✅

✅

✅

✅

DRAGEN RNA

[DragenRna_Settings]

Optional

DifferentialExpressionEnable

✅

✅

✅

✅

DRAGEN RNA

[DragenRna_Data]

Required

SampleID

✅

✅

✅

✅

DRAGEN RNA

[DragenRna_Data]

Required

ReferenceGenomeDir

✅

✅

✅

✅

DRAGEN RNA

[DragenRna_Data]

Optional

RnaGeneAnnotationFile

✅

✅

✅

✅

DRAGEN RNA

[DragenRna_Data]

Required

RnaPipelineMode

✅

✅

✅

✅

DRAGEN RNA

[DragenRna_Data]

Optional

DownSampleNumReads

✅

✅

✅

✅

DRAGEN RNA

[DragenRna_Data]

Optional

Comparison1

✅

✅

✅

✅

DRAGEN RNA

[DragenRna_Data]

Optional

Comparison2

✅

✅

✅

✅

DRAGEN RNA

[DragenRna_Data]

Optional

Comparison3

✅

✅

✅

✅

DRAGEN RNA

[DragenRna_Data]

Optional

Comparison4

✅

✅

✅

✅

DRAGEN RNA

[DragenRna_Data]

Optional

Comparison5

✅

✅

✅

✅

DRAGEN Somatic

[DragenSomatic_Settings]

Required

SoftwareVersion

✅

✅

DRAGEN Somatic

[DragenSomatic_Settings]

Required

AppVersion

✅

✅

DRAGEN Somatic

[DragenSomatic_Settings]

Required

MapAlignOutFormat

✅

✅

DRAGEN Somatic

[DragenSomatic_Settings]

Required

KeepFastq

✅

✅

DRAGEN Somatic

[DragenSomatic_Data]

Optional

AuxNoiseBaselineFile

✅

✅

DRAGEN Somatic

[DragenSomatic_Data]

Optional

AuxSvNoiseBaselineFile

✅

✅

DRAGEN Somatic

[DragenSomatic_Data]

Optional

AuxCnvPopBAlleleVcfFile

✅

✅

DRAGEN Somatic

[DragenSomatic_Data]

Optional

AuxGermlineTaggingFile

✅

✅

DRAGEN Somatic

[DragenSomatic_Data]

Required

VariantCallingMode

✅

✅

DRAGEN Somatic

[DragenSomatic_Data]

Required

Sample_ID

✅

✅

DRAGEN Methylation

[DragenMethylation_Settings]

Required

SoftwareVersion

✅

✅

DRAGEN Methylation

[DragenMethylation_Settings]

Required

AppVersion

✅

✅

DRAGEN Methylation

[DragenMethylation_Settings]

Required

MapAlignOutFormat

✅

✅

DRAGEN Methylation

[DragenMethylation_Settings]

Required

KeepFastq

✅

✅

DRAGEN Methylation

[DragenMethylation_Settings]

Required

UsesTaps

✅

✅

DRAGEN Methylation

[DragenMethylation_Data]

Required

ReferenceGenomeDir

✅

✅

DRAGEN Methylation

[DragenMethylation_Data]

Required

MethylationProtocol

✅

✅

DRAGEN Methylation

[DragenMethylation_Data]

Required

Sample_ID

✅

✅

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