Sequencer Auto-launch Analyses Compatibility

This page provides guidance on sequencer compatibility with different versions of DRAGEN secondary analysis pipelines and applications with auto-launch capabilities. This includes:

  • Pipelines with cloud analysis auto-launch. This type of workflow is available for BaseSpace and ICA users and includes automatic launch of secondary analysis in the cloud after sequencing completion.

  • Applications installed onboard of sequencers and on DRAGEN servers paired with sequencers. These type of applications allow you to configure settings for the run and analysis upfront and launch secondary analysis automatically after sequencing completes.

  • Tertiary analysis compatibility. Click on underlined versions in the table to view details on pipelines’ compatibility with Emedgene and Illumina Connected Insights.

This table does not cover all DRAGEN pipelines and applications. If a pipeline is not included in the table, it means that the auto-launch may not be available for it at all or not available for a specific instrument/pipeline combination. In this case, the pipeline could be used with the instrument if launched manually in BaseSpace, ICA or on the DRAGEN server. Non-DRAGEN pipelines are not included. Please check Local Run Manager materials and other resources on Illumina Support Center for additional onboard applications.

Pipeline

NextSeq 1000/2000

NextSeq 1000/2000

NovaSeq X Series

NovaSeq X Series

NovaSeq 6000 Dx (RUO mode)

NovaSeq 6000 Dx (RUO mode)

NovaSeq 6000

NextSeq 500/550/550 Dx (RUO mode)

MiSeq

MiniSeq

iSeq 100

Cloud

Onboard

Cloud

Onboard

Cloud

Paired server

Cloud

Cloud

Cloud

Cloud

Cloud

BCL Convert

v4.2.7 v3.10.4 v3.8.4 v3.7.4 v3.5.8 v3.5.6

v4.2.9

v4.2.7 v3.10.12 v3.10.11 v3.9.3 v3.8.4 v3.7.4 v3.5.8 v3.5.6

v4.1.23 v4.1.7

v4.1.23 v4.1.7 v4.1.5

v3.8.4

v3.9.5

v3.8.4

DRAGEN Germline

v3.5.8 v3.5.6

v3.5.8 v3.5.6

DRAGEN Enrichment

v3.5.8 v3.5.6

v3.5.8 v3.5.6

DRAGEN Amplicon

DRAGEN TruSight Oncology 500 ctDNA

DRAGEN RNA

v3.10.4 v3.8.4 v3.7.4 v3.5.8 v3.5.6

v3.10.12 v3.8.4 v3.7.4 v3.5.8 v3.5.6

DRAGEN Methylation

v4.1.23

v4.1.23

DRAGEN Single Cell RNA

v4.2.7 v3.10.4 v3.8.4 v3.7.4

v4.2.7 v3.10.12 v3.8.4 v3.7.4

DRAGEN Somatic

DRAGEN for DNA Prep with Enrichment

DRAGEN cfDNA Prep with Enrichment

NanoString GeoMx NGS Pipeline

v2.0.21

v2.0.21

DRAGEN COVIDSeq Test

v1.3.0

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