Sequencer Auto-launch Analyses Compatibility

This page provides guidance on sequencer compatibility with different versions of DRAGEN secondary analysis pipelines and applications with auto-launch capabilities. This includes:

• Pipelines with cloud analysis auto-launch. This type of workflow is available for BaseSpace and ICA users and includes automatic launch of secondary analysis in the cloud after sequencing completion.

• Applications installed onboard of sequencers and on DRAGEN servers paired with sequencers. These type of applications allow you to configure settings for the run and analysis upfront and launch secondary analysis automatically after sequencing completes.

• Tertiary analysis compatibility. Click on underlined versions in the table to view details on pipelines’ compatibility with Emedgene and Illumina Connected Insights.

This table does not cover all DRAGEN pipelines and applications. If a pipeline is not included in the table, it means that the auto-launch may not be available for it at all or not available for a specific instrument/pipeline combination. In this case, the pipeline could be used with the instrument if launched manually in BaseSpace, ICA or on the DRAGEN server. Non-DRAGEN pipelines are not included. Please check Local Run Manager materials and other resources on Illumina Support Center for additional onboard applications.

Pipeline	NextSeq 1000/2000	NextSeq 1000/2000	NovaSeq X Series	NovaSeq X Series	NovaSeq 6000 Dx (RUO mode)	NovaSeq 6000 Dx (RUO mode)	NovaSeq 6000	NextSeq 500/550/550 Dx (RUO mode)	MiSeq i100	MiSeq i100	MiSeq	MiniSeq	iSeq 100
	Cloud	Onboard	Cloud	Onboard	Cloud	Paired server	Cloud	Cloud	Cloud	Onboard	Cloud	Cloud	Cloud
BCL Convert	v4.2.7 v3.10.4 v3.8.4 v3.7.4 v3.5.8 v3.5.6	v4.2.9 v4.2.7 v3.10.12 v3.10.11 v3.9.3 v3.8.4 v3.7.4 v3.5.8 v3.5.6	v4.3.13 v4.1.23 v4.1.7	v4.3.13 v4.1.23 v4.1.7 v4.1.5	v3.8.4	v3.9.5	v3.8.4		v1.0.31	v1.0.31
DRAGEN Germline	v3.5.8 v3.5.6	v3.5.8 v3.5.6
DRAGEN Enrichment	v3.5.8 v3.5.6	v3.5.8 v3.5.6
DRAGEN Amplicon
DRAGEN TruSight Oncology 500 ctDNA
DRAGEN RNA	v3.10.4 v3.8.4 v3.7.4 v3.5.8 v3.5.6	v3.10.12 v3.8.4 v3.7.4 v3.5.8 v3.5.6
DRAGEN Methylation			v4.3.13 v4.1.23	v4.3.13 v4.1.23
DRAGEN Single Cell RNA	v4.2.7 v3.10.4 v3.8.4 v3.7.4	v4.2.7 v3.10.12 v3.8.4 v3.7.4
DRAGEN Somatic
DRAGEN for DNA Prep with Enrichment
DRAGEN cfDNA Prep with Enrichment
DRAGEN Protein Quantification			v1.8.33 v1.7.80
NanoString GeoMx NGS Pipeline	v2.0.21		v2.0.21
DRAGEN COVIDSeq Test	v1.3.0
DRAGEN Library QC									v4.3.13
DRAGEN Small WGS									v4.3.13
DRAGEN Microbial Enrichment Plus									v4.3.13