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On this page
  • Configure BaseSpace
  • Plan the Sequencing Run
  • Start the Sequencing Run
  • Monitor the Sequencing Run
  • View Sequencing Run Output
  • Monitor Secondary Analysis
  • View Secondary Analysis Results
  • Link Output Data to Other Projects

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  1. Cross-Product Tutorials

NovaSeq X: BCL Convert Auto-launch Analysis in Cloud

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Last updated 5 months ago

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This section provides a guided example of the end-to-end user flow for performing a sequencing run with data storage and automated analysis in ICA.

Configure BaseSpace

Plan the Sequencing Run

After selecting the appropriate user/workgroup context, navigate to the Runs tab in BaseSpace. Then click the New Run button and select Run Planning.

Set the Analysis Location to "BaseSpace/Illumina Connected Analytics" to configure for Cloud analysis.

Upon completion of the Run Planning inputs, the sequencing run can be either saved as a planned run in BaseSpace or exported as a Sample Sheet, depending on the instrument type.

Start the Sequencing Run

Follow the instructions for the sequencer to execute the planned sequencing run for cloud analysis. If the sequencer does not support consuming planned runs from BaseSpace, attach the sample sheet generated by Run Planning to a manual mode sequencing run.

Monitor the Sequencing Run

View Sequencing Run Output

Monitor Secondary Analysis

In ICA, the workflow session is launched and a record is present in the Analyses view in the project. The workflow session record can be identified by the User Reference, which will have "ws" preceding the run name.

Check the "status" of the workflow session to monitor progress in ICA. As orchestrated analyses are launched, analysis records will show in the Analyses view and in the workflow session details.

The workflow session details contain an "Orchestrated Analyses" section with the secondary analyses launched by the workflow session listed. Check the "status" of each analysis for progress. In the example below, a BCL Convert pipeline is launched by the workflow session.

The workflow session details also contain links to the BaseSpace Run Summary, which provides further information about the analyses, such as metrics, logs and reports.

View Secondary Analysis Results

The analysis output folder is stored in the ilmn-analyses folder in the project data view. The output folder name can be found in the Analysis Details view in the Output Files section. Filter for the correct output folder by copying the folder name from the Analysis Details view. The image below shows an example output folder from a BCL Convert automated analysis.

Post-sequencing automated analysis will typically include a demultiplex step using the Illumina-provided BCL Convert pipeline. The demultiplexed outputs from this pipeline result in the creation of project sample assets linked to associated demultiplexed data (ie, FASTQs).

Link Output Data to Other Projects

Follow instructions to configure sequencing run upload to ICA.

BaseSpace Run Planning provides an interface for planning a sequencing run. Only specific sequencing instruments support Basespace Run Planning. Refer to for details.

Navigate to and change to your desired workgroup or personal context. This may require you login to your domain first.

Use the guided Basespace Run Planning to create a planned run. See the documentation for details.

The configuration input forms vary depending on the instrument type, including the available secondary analysis applications (see for details). Subsequent configuration input forms vary depending on the secondary analysis application chosen. The images below demonstrate screens using a NovaSeq X Series instrument with Illumina DRAGEN BCL Convert analysis.

After a sequencing run is started, BaseSpace is used for monitoring the run through completion. Use the view to track the run progress.

Refer to for guidance on viewing the sequencing run data in ICA.

With analysis data available in the BaseSpace externally-managed project, the data can be linked to other ICA projects to serve as inputs for running pipelines, etc. Users with access to the BaseSpace externally-managed project can create links from the destination project Samples and Data views. See the and ICA documentation for details on linking sample data.

Sequencer Auto-launch Analyses Compatibility
BaseSpace
BaseSpace Run Planning
Sequencer Autolaunch Analyses Compatibility
BaseSpace Run Summary
Link Project Data
Link Project Sample
Analysis Auto-launch Tutorial
BaseSpace Setup
Sequencer Run Data in ICA