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DRAGEN Protein Quantification
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DRAGEN Protein Quantification
  • Get Started
    • Introduction
    • Prerequisites
      • Illumina Protein Prep Automation System Output Files
  • Run Setup
    • Run Planning with the BSSH Run Planner Tool
    • Sample Sheet Fields
    • Local Sample Sheet Generation Tool
    • Lane Splitting and Multi-Analysis by Project
  • Counting and Normalization
    • Cloud Autolaunch Secondary Analysis
      • Accessing Cloud Results
    • Local Secondary Analysis
    • Normalization Summary
    • QC Summary
    • Interpretation of Results
    • Metrics Appendix
  • Output Files
    • Output Structure
    • DRAGEN Report
    • ADAT Content
  • After Counting and Normalization
    • Using the ADAT
    • Illumina Connected Multiomics Walkthrough
  • References
    • FAQs
    • Documentation Revision History
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  1. Output Files

Output Structure

PreviousMetrics AppendixNextDRAGEN Report

Last updated 1 month ago

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DRAGEN Protein Quantification produces the following key output files in BaseSpace Sequence Hub:

  • DRAGEN_Protein_Quantification_<SW Version>

    • <project> (if no projects are provided, there shall be one folder titled with the RunName)

      • adat

        • <output file prefix>_FinalNormStep_MedNormExt.adat: This file contains the final normalized counts for the samples in this project.

        • other_normalization_steps: This folder contains all intermediate normalized ADATs with their respective counts.

      • quality metrics

        • <output file prefix>_run_qc_stats.csv: This file provides a summary of per sample run qc statistics.

      • DRAGEN Report

  • DRAGEN Report: Folder containing all projects DRAGEN report file/s

  • Extra Files

  • ICA Logs: Folder containing information on ICA logs

Example of Output Structure in BSSH

The example below contains an analysis configured with two Projects, "1_x" and "0_x". Analysis and outputs are processed for each project individually (check for details).

Multi-Analysis section
Each project folder (highlighted in yellow) includes "quality_metrics" and "adat" folders, along with a DRAGEN Report. The final normalized counts file (FinalNormStep_MedNormExt.adat) is available for each project and can be used in exploratory analysis.