LogoLogo
Illumina KnowledgeIllumina SupportSign In
DRAGEN Protein Quantification
  • Home
DRAGEN Protein Quantification
  • Get Started
    • Introduction
    • Prerequisites
      • Illumina Protein Prep Automation System Output Files
  • Run Setup
    • Run Planning with the BSSH Run Planner Tool
    • Sample Sheet Fields
    • Local Sample Sheet Generation Tool
    • Lane Splitting and Multi-Analysis by Project
  • Counting and Normalization
    • Cloud Autolaunch Secondary Analysis
      • Accessing Cloud Results
    • Local Secondary Analysis
    • Normalization Summary
    • QC Summary
    • Interpretation of Results
    • Metrics Appendix
  • Output Files
    • Output Structure
    • DRAGEN Report
    • ADAT Content
  • After Counting and Normalization
    • Using the ADAT
    • Illumina Connected Multiomics Walkthrough
  • References
    • FAQs
    • Documentation Revision History
Powered by GitBook
On this page
  • Prerequisites
  • Steps
  • Proteomics Sample Sheet Generator

Was this helpful?

Export as PDF
  1. Run Setup

Local Sample Sheet Generation Tool

PreviousSample Sheet FieldsNextLane Splitting and Multi-Analysis by Project

Last updated 1 month ago

Was this helpful?

The Run Planning web interface, accessible via , requires internet connection. The Proteomics Sample Sheet Generator tool (linked below) allows for offline creation of sample sheets compatible with the local DRAGEN Protein Quantification application.

Prerequisites

  1. Local Proteomics Sample Sheet Generator Excel Workbook.

  2. One or more IPPAS output files (one per plate).

  3. Access to a DRAGEN Server configured to the Illumina Protein Quantification Local Secondary Analysis (see prerequisites page for more information).

Steps

  1. Download and open the Local Proteomics Sample Sheet Generator excel file.

  2. Navigate to the Start tab and follow the instructions described in the upper left portion of the sheet:

    1. Fill in the RunName and output_file_prefix.

    2. Change the InstrumentPlatform and PrepKitName as needed.

    3. Copy and paste values from IPPAS output file(s) not including the headers into the specified fields.

    4. Add the Flow Cell lanes for each sample as a comma separated list with no spaces as needed.

    5. Ensure there are no errors displayed in the Row Check or Overall Check (a green cell is expected)

    6. Navigate to the "SaveAsCSV" and save it as CSV (filename must not contain special characters).

    7. This is a sample sheet compatible to local DRAGEN Protein Quantification.

  3. (Recommended) Load this sample sheet onto the sequencer prior to sequencing.

    1. If the sample sheet is not included prior to sequencing, the user must manually reference the sample sheet when running DRAGEN Protein Quantification later.

  4. Analyze the data locally using a DRAGEN Server with DRAGEN Protein Quantification Pipeline (see for more information).

Proteomics Sample Sheet Generator

Base Space Sequence Hub (BSSH)
Local Secondary Analysis page
524KB
ProteomicsSamplesheetGenerator.xlsx