Introduction to DRAGEN TSO 500 ctDNA Analysis Software v2.6
The Illumina DRAGEN TruSight Oncology 500 (TSO 500) ctDNA Analysis Software supports analysis for DNA libraries that are isolated from plasma and prepared using TruSight Oncology 500 ctDNA v2 and v1 assays. The software, which can be run on the DRAGEN Server, on Illumina Connected Analytics, and as an analysis application on NovaSeq 6000Dx, produces a variant call file (VCF) for small variants. Other outputs include tumor mutational burden (TMB), a Jensen-Shannon divergence (sum JSD) score that can be used for evaluating microsatellite instability (MSI) status, files with copy number variants (CNV), fusions as well as coverage reports.
The secondary analysis software starts from a sequencing run folder containing base call files (BCL) or from FASTQ files staged in a FASTQ folder.
This document provides information on installation, configuration, running, troubleshooting as well as analysis algorithms of DRAGEN TruSight Oncology 500 ctDNA analysis software on Illumina Connected Analytics, standalone DRAGEN server, and the NovaSeq 6000Dx analysis application.
The software is optimized for analyzing sequencing outputs generated by the TruSight Oncology 500 ctDNA v2 and v1 assays. Modification might lead to inaccurate data and is a violation of the Illumina Software Subscription Agreement.
Scope
This resource provides information on installation, configuration, running, troubleshooting and analysis algorithms for the following software:
DRAGEN TruSight Oncology 500 ctDNA Analysis Software on Illumina Connected Analytics (ICA)
v2.6.0
v2.6.1
DRAGEN TruSight Oncology 500 ctDNA Analysis Software (for standalone DRAGEN server)
v2.6.0
v2.6.1
v2.6.2
DRAGEN TruSight Oncology 500 ctDNA Analysis Application on NovaSeq 6000Dx (uses a paired DRAGEN server)
v2.6.0
The content is applicable to all 3 software versions unless otherwise specified. The content related to setting up and running the analysis on ICA is only relevant to software versions v2.6.0 and v2.6.1. The content related to the analysis application on NovaSeq 6000Dx is only relevant to v2.6.1.
Local and Cloud Deployments
Local analysis is available using a standalone DRAGEN server or an application with a user interface on NovaSeq 6000Dx. The software on the standalone DRAGEN server allows for analysis on a single DRAGEN server or splitting across multiple servers.
Cloud analysis is available on Illumina Connected Analytics with auto-launch or manual launch. Both methods are available from BCLs and FASTQs.
Instrument Compatibility
DRAGEN TruSight Oncology 500 ctDNA analysis software is compatible with data generated on the Illumina instruments as summarized in the table below.
NovaSeq 6000
Yes (v2.6.0 and v2.6.1)
Yes
N/A
N/A
NovaSeq 6000Dx (RUO mode)
Yes (v2.6.0 and v2.6.1)
Yes
Yes (v2.6.1)
N/A
NovaSeq X
Yes (v2.6.0 and v2.6.1)
Yes
N/A
No
Navigation of Guide
This resource provides information on installation, configuration, running, troubleshooting as well as analysis algorithms of DRAGEN TruSight Oncology 500 ctDNA analysis software on Illumina Connected Analytics, standalone DRAGEN server, and the NovaSeq 6000Dx analysis application.
Workflow Diagram
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