Installation of NovaSeq 6000Dx TSO 500 Analysis Application

Instructions to install DRAGEN TSO 500 Analysis Application on NovaSeq 6000Dx (RUO mode).

Prerequisites

• A NovaSeq 6000Dx sequencing instrument with paired DRAGEN server v4

• Illumina Run Manager installed by Illumina support personnel

• TSOCombined and TSO500_HRD licenses installed by Illumina support personnel

  • TSOCombined and TSO500_HRD licenses are pre-installed in manufacturing since February 2025 and will need to be installed only for instruments purchased prior to that

• The user installing the app must have admin privileges on Illumina Run Manager

Installation Instructions:

1. Contact Illumina Customer Care at [e-mail] to obtain installation package for Illumina DRAGEN TruSight Oncology 500 (HRD) Analysis Application on NovaSeq 6000Dx

2. Download the installation package provided in the email by Illumina Customer Care. The link will expire after 7 days.

It is recommended to use a command line tool like wget or curl to download the file rather than pasting the link into the web browser bar. For example:

curl -o {filename} "{link}"

wget -O {filename} '{link}'

Where the file name is the name of either DRAGEN TSO 500 Analysis Application file or DRAGEN ires file, and the link is provided by Illumina Customer Care.

1. The installation package contains the following:

   1. DRAGEN TSO 500 Analysis Application: DRAGEN_TSO500HRD_v2.6.0-2v12.iapp

   2. DRAGEN ires: drageninstaller_3.10.17-8.el8.x86_64_prod.ires

MD5sum for the installation package contents are as follows:

• drageninstaller_3.10.17-8.el8.x86_64_prod.ires

  • 12df06502776d8b673c73fb714dc466a

• DRAGEN_TSO500HRD_v2.6.0-2v12.iapp

  • dc4fe5fe2fc3eca57969e978886dcedf

1. Install the DRAGEN version using the ires:

   1. Log into Illumina Run Manager as a user with admin credentials

   2. Navigate to the top left menu to "DRAGEN" in the drop down

   3. Select "Add DRAGEN Installer" and upload the DRAGEN ires file

   4. The installation is complete once the DRAGEN version 3.10.17 is in the install version(s) list

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