DRAGEN Applications
Applications
DRAGEN analysis offers a large selection of application pipelines.
| Pipeline | Description | Variant Types Detected | Metrics Provided |
|---|---|---|---|
DRAGEN Demultiplexing | Rapid demultiplexing of NGS analysis | N/A | N/A |
DRAGEN ORA Compression | DRAGEN ORA compression is optimized for high compression ratios of FASTQ files, as well as rapid compression and decompression, all while preserving data integrity. | N/A | Compression Ratio Run Time |
DRAGEN Map + Align | The DRAGEN Map + Align can be run as a standalone or as part of DRAGEN’s suite of pipelines | N/A | Mapping metrics Duration Metrics Coverage Metrics |
DRAGEN Germline | The DRAGEN Germline Pipeline provides end-to-end NGS analysis, including advanced error model calibration for increased accuracy, and repeat expansion detection and genotyping through Illumina Expansion Hunter. | SNV/Indel CNV SV Repeat Expansions | Mapping metrics Duration Metrics Coverage Metrics Variant Metrics Callability Report |
DRAGEN Somatic | The DRAGEN Somatic Pipeline includes tumor-only and tumor–normal modes, designed for detecting somatic variants in tumor samples. Both modes make no ploidy assumptions, enabling detection of low-frequency alleles. | SNV/Indel CNV SV TMB MSI HLA | Mapping metrics Duration Metrics Coverage Metrics Variant Metrics Callability Report |
DRAGEN Enrichment | The DRAGEN Enrichment Pipeline combines DRAGEN’s germline and somatic callers into a pipeline designed specifically for analyzing enrichment samples. Includes a full suite of enrichment metrics and reporting. | SNV/Indel CNV SV | Mapping metrics Duration Metrics Coverage Metrics Variant Metrics Callability Report |
DRAGEN RNA | The DRAGEN RNA Pipeline performs transcriptome analysis starting with splice junction discovery and alignment, followed by rapid alignment and splice junction mapping and quantification. For differential expression, Illumina recommends the DRAGEN Differential Expression app on BaseSpace Sequence Hub. | Gene fusion SNV/Indel | Mapping metrics Duration Metrics Coverage Metrics Variant Metrics Callability Report |
DRAGEN Single Cell RNA | The DRAGEN Single Cell RNA pipeline performs demultiplexing, cell-barcode and UMI error correction, sequence alignment, and quantification of gene expression. | N/A | Mapping Metrics Duration Metrics Coverage Metrics Callability Report Cell Metrics |
DRAGEN Joint Genotyping | The DRAGEN Joint Genotyping/Population Pipeline calls variants jointly across multiple genomes and scales to large cohorts of samples at expedited speeds with uncompromising accuracy. | SNV/Indel CNV SV Repeat Expansions | Mapping metrics Duration Metrics Coverage Metrics Variant Metrics Callability Report |
DRAGEN Methylation | The DRAGEN Methylation Pipeline performs alignment, methyl calling, and calculates alignment and methylation metrics. | N/A | Mapping metrics Duration Metrics Coverage Metrics Variant Metrics Callability Report |
DRAGEN Reference Builder | Accepts FASTA files, and builds the proprietary reference used by the DRAGEN apps. | N/A | N/A |
DRAGEN TruSight Oncology 500 ctDNA Analysis Software | Secondary analysis support for Illumina’s TruSight Oncology 500 ctDNA. Available on the local DRAGEN Server version 3 and later. | SNV/Indel CNV DNA fusions MSI TMB | Mapping metrics Duration Metrics Coverage Metrics Variant Metrics Callability Report |
DRAGEN Imputation | The DRAGEN Imputation pipeline is an end to end user friendly tool that enables scalable low pass whole genome sequencing analysis | N/A | Impute ≤100 samples simultaneously 1.7x faster compared to original GLIMPSE code |
Analysis Uses
DRAGEN analysis can be used in numerous fields in the biological sciences.
| Analysis | Description |
|---|---|
Genetic Diseases | Reduce time required for genomic analysis, with high accuracy and comprehensiveness |
Oncology | Analyze tumor-only and tumor/normal samples with accuracy, comprehensiveness, and efficiency |
Cell and Molecular Biology | Advance understanding of cellular mechanisms with rapid analysis pipelines for bulk and single cell samples |
Population Genomics | Accurately and efficiently analyze sequenced genomes at scale. Accelerate re-analysis as computational tools improve over time |
Infectious Disease | Detect and characterize infectious diseases with a comprehensive solution |
Agrigenomics | Efficiently analyze animals and plants of varying genomic complexities with custom reference |
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