DRAGEN on AWS

You can run DRAGEN analysis on Amazon Web Services (AWS). For information on using DRAGEN, see the DRAGEN User Guide Section. For information on using AWS, see the AMI documentation available on the Amazon Web Services site.

You must have access to a DRAGEN license to use DRAGEN on AWS. Contact Illumina Technical Support to obtain a DRAGEN license.

Launch an Instance

1. Navigate to the AWS Management Console, and then sign in.

2. Select the Services menu on the top toolbar, and then select EC2 under Compute.

3. Under Images, select AMIs.

4. Select the AMI ID of your instance.

5. Launch the image.

6. Select the instance type, and then select Next to configure additional VM settings. The f1.4xlarge instance type is recommended for DRAGEN. For more information on instance settings, see the AMI documentation available on the Amazon Web Services site.

7. After you have finished, select Review and Launch.

8. Select Choose an existing key pair or Proceed without a key pair, and then select Launch Instance.

9. When the launch confirmation page displays, select View Instances to navigate to your list of instances.

Configure Your Instance

1. Navigate to the Instances page, and then select the instance to connect to.

2. Select Connect.

3. Select SSH client and connect to the instance. For more information on connecting to an instance, see the AMI documentation available on the Amazon Web Services site.

4. Mount the disks to /staging on the instance using the following commands.

sudo yum -y install mdadm
sudo mdadm --create --verbose /dev/md0 --level=0 --name=MY_RAID0 --raid-devices=<number of volumes> <device name 1> <device name 2>
sudo mkfs.ext4 -L MY_RAID0 /dev/md0
sudo mkdir -p /staging
sudo sh -c "echo 'LABEL=MY_RAID0 /staging ext4 defaults,noatime 0 0' >> /etc/fstab"
sudo mount -a

For example, the following command mounts four volumes attached at /dev/xvdb, /dev/xvdc, /dev/xvdd, and /dev/xvde to a RAID 0 on /staging.

sudo yum -y install mdadm
sudo mdadm --create --verbose /dev/md0 --level=0 --name=MY_RAID0 --raid-devices=4 /dev/xvdb /dev/xvdc /dev/xvdd /dev/xvde
sudo mkfs.ext4 -L MY_RAID0 /dev/md0
sudo mkdir -p /staging
sudo sh -c "echo 'LABEL=MY_RAID0 /staging ext4 defaults,noatime 0 0' >> /etc/fstab"
sudo mount -a

You can use DRAGEN command-line options. For more information on DRAGEN analysis and command line options, see the Command Line Options Section of the user guide. For information on using AWS, see the AMI documentation available on the Amazon Web Services site.